Prop J, Griffiths A, Hutchinson I V, Morris P J
Cell Immunol. 1986 Apr 15;99(1):73-84. doi: 10.1016/0008-8749(86)90217-0.
The optimal conditions for the induction of contact hypersensitivity in rats and the characteristics of its suppression were studied using the sensitizing haptens dinitrofluorobenzene (DNFB) and trinitrochlorobenzene (TNCB). The hypersensitivity was shown to be hapten specific in so far as TNCB did not sensitize for DNFB responses but sensitization with DNFB did allow a marginal response in rats challenged with TNCB. Suppression of the sensitization to DNFB and TNCB could be generated by intravenous injection of dinitrobenzenesulphonic acid (DNBS) or trinitrobenzenesulphonic acid (TNBS), respectively, up to 3 weeks before sensitization. This suppression was hapten specific and could be transferred with splenic T cells enriched for lymphocytes carrying the OX8 (Tc/s) cell marker. Only the induction phase of sensitization, however, could be suppressed in that way. No suppression acting upon the effector phase could be detected except for a nonspecific local suppression at the site of a previous challenge with an antigen to which the rat was specifically suppressed. This study shows that suppression of contact hypersensitivity in rats is mediated by specific suppressor T cells of which the activation pathway apparently differs from that postulated for mice.
利用致敏半抗原二硝基氟苯（DNFB）和三硝基氯苯（TNCB），研究了大鼠接触性超敏反应诱导的最佳条件及其抑制特性。结果表明，超敏反应具有半抗原特异性，因为TNCB不能使大鼠对DNFB反应产生致敏，但用DNFB致敏确实能使大鼠在受到TNCB攻击时产生轻微反应。在致敏前长达3周，分别静脉注射二硝基苯磺酸（DNBS）或三硝基苯磺酸（TNBS），可产生对DNFB和TNCB致敏的抑制作用。这种抑制作用具有半抗原特异性，并且可以通过富含携带OX8（Tc/s）细胞标记的淋巴细胞的脾T细胞进行转移。然而，只有致敏的诱导阶段可以通过这种方式被抑制。除了在大鼠先前被特异性抑制的抗原攻击部位存在非特异性局部抑制外，未检测到对效应阶段的抑制作用。这项研究表明，大鼠接触性超敏反应的抑制是由特异性抑制性T细胞介导的，其激活途径显然不同于小鼠的假定途径。
