Prop J, Hutchinson I V, Morris P J
Cell Immunol. 1986 Apr 15;99(1):85-94. doi: 10.1016/0008-8749(86)90218-2.
The shift of suppression from a tolerizing hapten to a so-called bystander antigen was investigated in this study using contact hypersensitivity to trinitrochlorobenzene (TNCB) and dinitrofluorobenzene (DNFB) and delayed type hypersensitivity (DTH) to alloantigens in the rats as experimental models. Primary suppression of contact hypersensitivity was induced by intravenous injection of the water-soluble forms of TNCB and DNFB. A shift of the suppression to the bystander hapten was found if the tolerizing and bystander hapten were mixed and applied to the same area of skin during the sensitization procedure, but not if they were applied to separate areas of skin. With alloantigens, bystander suppression developed only when the sensitizing allogeneic cells were mixed with hapten-modified syngeneic cells. It was not induced by hapten-modified allogeneic cells. Once induced, such bystander suppression of the response to haptens persisted independently of the primarily tolerizing hapten, and it could be adoptively transferred with spleen cells. These results favour the concept that the bystander suppression is mediated by the non-specific action of suppressor cells generated specifically during the mixed sensitization rather than by an antigen bridge.
本研究以大鼠对三硝基氯苯（TNCB）和二硝基氟苯（DNFB）的接触性超敏反应以及对同种异体抗原的迟发型超敏反应（DTH）作为实验模型，研究了抑制作用从耐受性半抗原向所谓旁观者抗原的转移。通过静脉注射水溶性形式的TNCB和DNFB诱导接触性超敏反应的初级抑制。如果在致敏过程中将耐受性半抗原和旁观者半抗原混合并应用于皮肤的同一区域，则会发现抑制作用向旁观者半抗原转移，但如果将它们应用于皮肤的不同区域则不会。对于同种异体抗原，仅当致敏的同种异体细胞与半抗原修饰的同基因细胞混合时才会产生旁观者抑制。它不是由半抗原修饰的同种异体细胞诱导的。一旦诱导产生，这种对半抗原反应的旁观者抑制会独立于最初耐受性半抗原而持续存在，并且可以通过脾细胞进行过继转移。这些结果支持这样一种观点，即旁观者抑制是由混合致敏过程中特异性产生的抑制细胞的非特异性作用介导的，而不是由抗原桥介导的。
