Ghanbarzadeh S, Garjani A, Ziaee M, Khorrami A
Research Center for Pharmaceutical Nanotechnology and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Pharmacology & Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Res (Stuttg). 2014 Sep;64(9):449-53. doi: 10.1055/s-0033-1361103. Epub 2013 Nov 27.
Nowadays, cardiovascular diseases and male infertility are two big health problems in industrial countries.The aim of the present study was to investigate the protective role of coenzyme Q10 and L-Carnitine pretreatment in the impaired spermatogenesis caused by isoproterenol (ISO) in male rats.Thirty-two male Wistar rats were allocated in 4 groups. ISO was injected for 2 consecutive days (100 mg/kg) in ISO treated groups. Before ISO administration, pretreatment with Coenzyme Q10 (10 mg/kg/day) and L-Carnitine (350 mg/kg/day) were conducted for 20 consecutive days. Sex hormones level, malondialdehyde (MDA) and total antioxidant concentration as well as testis, epididymis and seminal vesicle weight were investigated.Increase in the concentration of MDA and decrease in total antioxidant level was observed following ISO administration. Accordingly, the sperm viability as well as testis, epididymis and seminal vesicle weights were decreased. In the case of sex hormones, the testosterone and LH levels were decreased and the concentration of FSH was increased. Pretreatment with L-carnitine and Coenzyme Q10 significantly decreased the MDA level and increased total antioxidant, LH and testosterone levels. Pretreatment with L-carnitine and Coenzyme Q10 also improved semen parameters and organs weight which were impaired by ISO administration.L-carnitine and Coenzyme Q10 pretreatment could protect spermatogenesis in male rats with ISO administration.
如今，心血管疾病和男性不育是工业化国家面临的两大健康问题。本研究的目的是探讨辅酶Q10和L-肉碱预处理对异丙肾上腺素（ISO）所致雄性大鼠精子发生受损的保护作用。32只雄性Wistar大鼠被分为4组。ISO处理组连续2天注射ISO（100mg/kg）。在给予ISO之前，连续20天用辅酶Q10（10mg/kg/天）和L-肉碱（350mg/kg/天）进行预处理。检测性激素水平、丙二醛（MDA）和总抗氧化剂浓度，以及睾丸、附睾和精囊重量。给予ISO后，MDA浓度升高，总抗氧化剂水平降低。相应地，精子活力以及睾丸、附睾和精囊重量均下降。就性激素而言，睾酮和促黄体生成素（LH）水平降低，促卵泡生成素（FSH）浓度升高。L-肉碱和辅酶Q10预处理显著降低了MDA水平，提高了总抗氧化剂、LH和睾酮水平。L-肉碱和辅酶Q10预处理还改善了因给予ISO而受损的精液参数和器官重量。L-肉碱和辅酶Q10预处理可保护给予ISO的雄性大鼠的精子发生。
