Asl Fatemeh Rahimi, Khosravi Maryam, Hajikhani Ramin, Solati Jalal, Fahimi Hossein
Department of Biology, Islamic Azad University North Tehran Branch, Tehran, Iran.
Department of Biology, Faculty of Biological Sciences, Islamic Azad University North Tehran Branch, Tehran, Iran.
Int J Reprod Biomed. 2021 Aug 16;19(7):607-618. doi: 10.18502/ijrm.v19i7.9471. eCollection 2021 Jul.
Coenzyme Q10 (CoQ10) and (LS) have therapeutic effects on infertility.
To evaluate the combined effects of LS and CoQ10 on reproductive function in adult male NMRI mice.
Eighty three-months-old male mice (35-40 gr) were divided into four groups (n = 10/each): control (treated with water), CoQ10-treated (200, 300, and 400 mg/kg/body weight), LS-treated (200, 400, 600 mg/kg/body weight), and co-treated (LS [600 mg/kg/body weight] + CoQ10 [200 mg/kg/body weight]) groups. Serum testosterone, luteinizing hormone, follicle-stimulating hormone, and gonadotropin realizing hormone (GnRH) levels were measured using ELISA method. The sperm quality was assessed using Sperm Class AnalyzerⓇ (SCA) CASA system and GnRH mRNA expression levels were evaluated by real-time polymerase chain reaction.
The number of sniffing and following behavior was significantly higher in LS-treated (400 and 600 mg/ml/body weight) groups than the control group (p = 0.0007 and p = 0.0010, respectively). The number of mounting and coupling behaviors was significantly higher in the CoQ10 (300 and 400 mg/ml/body weight)-treated animals than the control group (p = 0.0170 and p = 0.0006, respectively). Co-treatment of CoQ10 (200 mg/ml/body weight) and LS (600 mg/ml/body weight) significantly increased all aspects of sexual behaviors as well as the levels of serum testosterone (p = 0.0011), luteinizing hormone (p = 0.0062), and follicle-stimulating hormone (p = 0.0001); sperm viability (p = 0.0300) and motility (p = 0.0010); and GnRH mRNA levels (p = 0.0016) compared to the control group.
The coadministration of CoQ10 and LS significantly improves the activity of the hypothalamic-pituitary-gonadal axis and enhances the reproductive parameters in adult male mice.
辅酶Q10（CoQ10）和左旋肉碱（LS）对不孕症具有治疗作用。
评估LS和CoQ10联合应用对成年雄性NMRI小鼠生殖功能的综合影响。
将80只3月龄雄性小鼠（体重35 - 40克）分为四组（每组n = 10）：对照组（给予水）、CoQ10处理组（200、300和400毫克/千克体重）、LS处理组（200、400、600毫克/千克体重）以及联合处理组（LS[600毫克/千克体重]+CoQ10[200毫克/千克体重]）。采用酶联免疫吸附测定法（ELISA）检测血清睾酮、黄体生成素、卵泡刺激素和促性腺激素释放激素（GnRH）水平。使用精子分类分析仪Ⓡ（SCA）计算机辅助精子分析（CASA）系统评估精子质量，并通过实时聚合酶链反应评估GnRH mRNA表达水平。
LS处理组（400和600毫克/毫升/体重）的嗅探和跟随行为次数显著高于对照组（分别为p = 0.0007和p = 0.0010）。CoQ10处理组（300和400毫克/毫升/体重）的爬跨和交配行为次数显著高于对照组（分别为p = 0.0170和p = 0.0006）。CoQ10（200毫克/毫升/体重）和LS（600毫克/毫升/体重）联合处理显著增加了性行为的各个方面以及血清睾酮水平（p = 0.0011）、黄体生成素水平（p = 0.0062）和卵泡刺激素水平（p = 0.0001）；精子活力（p = 0.0300）和运动能力（p = 0.0010）；与对照组相比，GnRH mRNA水平也显著增加（p = 0.0016）。
CoQ10和LS联合给药显著改善成年雄性小鼠下丘脑 - 垂体 - 性腺轴的活性，并提高生殖参数。
