褪黑素和β-葡聚糖对大鼠肝损伤的保护作用——一项对比研究。
Protective effects of melatonin and β-d-glucan against liver injury in rats - a comparative study.
机构信息
Department of Anesthesiology and Reanimation, Inonu University, Faculty of Medicine, Malatya, Turkey.
出版信息
Adv Clin Exp Med. 2013 Sep-Oct;22(5):621-7.
OBJECTIVES
The aim of this study was to investigate the possible protective effects of melatonin and β-d-glucan against ischemia-reperfusion (IR) injury in rats.
MATERIAL AND METHODS
Forty rats were randomly divided into 5 groups, each consisting of 8 animals, as follows. Sham group [S], IR group [C], IR + β-Glucan group [β], IR + melatonin group [MLT], IR + melatonin + β-Glucan group [MLT + β]. The rats in the C, β, MLT and MLT + β groups were subjected to IR for 60 min each. Melatonin (10 mg∙kg⁻¹) was intraperitoneally injected for a single dose 30 min before IR. β-Glucan (50 mg∙kg⁻¹∙day⁻¹) was orally administered for 10 days to rats. All of the rats were killed on day 11, and histological changes in the liver and tissue levels of oxidants and antioxidants were evaluated.
RESULTS
Malondialdehyde [MDA] level were significantly higher in the C group compared to the S group (p = 0.007). MDA level were significantly higher in the β group compared to the MLT and MLT + β groups (p =0.007). Tissue antioxidant markers (superoxide dismut ase [SOD], glutathione-peroxidase [GPx], and catalase [CAT]) were significantly lower in the C group than the S group (p < 0.05). SOD levels were simply not significant in the β group compared to the MLT and MLT + β groups. CAT and GPx activities were significantly higher in the β group compared to the MLT and MLT + β groups (p = 0.004).The histological damage ameliorated in β, MLT and MLT + β groups compared to C group.
目的
本研究旨在探讨褪黑素和β-D-葡聚糖对大鼠缺血再灌注（IR）损伤的可能保护作用。
材料和方法
40 只大鼠随机分为 5 组，每组 8 只，如下。假手术组[S]、IR 组[C]、IR+β-葡聚糖组[β]、IR+褪黑素组[MLT]、IR+褪黑素+β-葡聚糖组[MLT+β]。C、β、MLT 和 MLT+β 组大鼠均接受 60minIR。IR 前 30min 腹腔内注射褪黑素（10mg·kg⁻¹）单次剂量。β-葡聚糖（50mg·kg⁻¹·天⁻¹）灌胃给药，连续 10 天。所有大鼠于第 11 天处死，评估肝组织学变化和组织氧化应激标志物及抗氧化剂水平。
结果
与 S 组相比，C 组丙二醛[MDA]水平显著升高（p=0.007）。与 MLT 和 MLT+β 组相比，β 组 MDA 水平显著升高（p=0.007）。与 S 组相比，C 组组织抗氧化标志物（超氧化物歧化酶[SOD]、谷胱甘肽过氧化物酶[GPx]和过氧化氢酶[CAT]）水平显著降低（p<0.05）。与 MLT 和 MLT+β 组相比，β 组 SOD 水平无显著性差异。与 MLT 和 MLT+β 组相比，β 组 CAT 和 GPx 活性显著升高（p=0.004）。与 C 组相比，β、MLT 和 MLT+β 组的组织学损伤得到改善。
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