Department of Chemistry, Yonsei University, Seoul 120-749, Korea.
Chem Commun (Camb). 2013 Sep 18;49(72):7959-61. doi: 10.1039/c3cc44627a. Epub 2013 Jul 31.
A new rhodamine fluorescent probe for monitoring ˙OH has been developed based on the oxidative C-H abstraction reaction of rhodamine cyclic hydrazide. The probe exhibits excellent selectivity for ˙OH with virtually no interference by other ROS/RNS species. Fluorescent imaging of A549 and RAW264.7 cells is also successfully demonstrated to detect intracellular ˙OH in live cells.
一种新的用于监测·OH 的罗丹明荧光探针已基于罗丹明环酰肼的氧化 C-H 抽离反应而开发。该探针对·OH 具有优异的选择性，几乎不受其他 ROS/RNS 物质的干扰。还成功地进行了 A549 和 RAW264.7 细胞的荧光成像，以在活细胞中检测细胞内的·OH。
