Carvalho Ana, Paiva Jorge, Louzada José, Lima-Brito José
Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB/CGB), University of Tras-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal.
Mol Biol Int. 2013;2013:974324. doi: 10.1155/2013/974324. Epub 2013 Oct 29.
In the last years, forestry scientists have adapted genomics and next-generation sequencing (NGS) technologies to the search for candidate genes related to the transcriptomics of secondary growth and wood formation in several tree species. Gymnosperms, in particular, the conifers, are ecologically and economically important, namely, for the production of wood and other forestry end products. Until very recently, no whole genome sequencing of a conifer genome was available. Due to the gradual improvement of the NGS technologies and inherent bioinformatics tools, two draft assemblies of the whole genomes sequence of Picea abies and Picea glauca arose in the current year. These draft genome assemblies will bring new insights about the structure, content, and evolution of the conifer genomes. Furthermore, new directions in the forestry, breeding and research of conifers will be discussed in the following. The identification of genes associated with the xylem transcriptome and the knowledge of their regulatory mechanisms will provide less time-consuming breeding cycles and a high accuracy for the selection of traits related to wood production and quality.
在过去几年中,林业科学家已将基因组学和下一代测序(NGS)技术应用于多种树种中与次生生长和木材形成转录组学相关的候选基因搜索。裸子植物,特别是针叶树,在生态和经济方面都很重要,尤其是在木材和其他林业终端产品的生产方面。直到最近,还没有针叶树基因组的全基因组测序。由于NGS技术和固有生物信息学工具的逐步改进,当年出现了欧洲云杉和白云杉全基因组序列的两个草图组装。这些基因组草图组装将为针叶树基因组的结构、内容和进化带来新的见解。此外,以下将讨论针叶树林业、育种和研究的新方向。与木质部转录组相关的基因鉴定及其调控机制的知识将提供耗时更少的育种周期,并为与木材生产和质量相关的性状选择提供高精度。