Liu Jing-Wei, He Cai-Yun, Sun Li-Ping, Xu Qian, Xing Cheng-Zhong, Yuan Yuan
Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Affiliated Hospital of China Medical University, and Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Education Department, Shenyang, China E-mail :
Asian Pac J Cancer Prev. 2013;14(10):6103-8. doi: 10.7314/apjcp.2013.14.10.6103.
Excision repair cross-complementing group 6 (ERCC6) is a major component of the nucleotide excision repair pathway that plays an important role in maintaining genomic stability and integrity. Several recent studies suggested a link of ERCC6 polymorphisms with susceptibility to various cancers. However, the relation of ERCC6 polymorphism with gastric cancer (GC) risk remains elusive. In this sex- and age- matched case-control study including 402 GC cases and 804 cancer-free controls, we aimed to investigate the association between a potentially functional polymorphism (rs1917799 T>G) in the ERCC6 regulatory region and GC risk.
The genotypes of rs1917799 were determined by Sequenom MassARRAY platform and the status of Helicobacter pylori infection was detected by enzyme-linked immunosorbent assay. Odd ratios (ORs) and 95% confidential interval (CI) were calculated by logistic regression analysis.
Compared with the common TT genotype, the ERCC6 rs1917799 GG genotype was associated with increased GC risk (adjusted OR=1.46, 95%CI: 1.03-2.08, P=0.035). When compared with (GT+TT) genotypes, the GG genotype also demonstrated a statistical association with increased GC risk (adjusted OR=1.38, 95%CI: 1.01-1.89, P=0.044). This was also observed for the male subpopulation (GG vs. TT: adjusted OR=1.71, 95%CI: 1.12-2.62, P=0.013; G allele vs. T allele: adjusted OR=1.32, 95%CI: 1.07-1.62, P=0.009). Genetic effects on increased GC risk tended to be enhanced by H. pylori infection, smoking and drinking, but their interaction effects on GC risk did not reach statistical significance.
ERCC6 rs1917799 GG genotype might be associated with increased GC risk in Chinese, especially in males.
切除修复交叉互补基因6（ERCC6）是核苷酸切除修复途径的主要组成部分，在维持基因组稳定性和完整性方面发挥重要作用。最近的几项研究表明ERCC6基因多态性与多种癌症的易感性有关。然而，ERCC6基因多态性与胃癌（GC）风险之间的关系仍不明确。在这项包括402例GC病例和804例无癌对照的性别和年龄匹配的病例对照研究中，我们旨在研究ERCC6调控区一个潜在功能性多态性（rs1917799 T>G）与GC风险之间的关联。
采用Sequenom MassARRAY平台测定rs1917799的基因型，采用酶联免疫吸附试验检测幽门螺杆菌感染状况。通过逻辑回归分析计算比值比（OR）和95%置信区间（CI）。
与常见TT基因型相比，ERCC6 rs1917799 GG基因型与GC风险增加相关（校正OR=1.46，95%CI：1.03-2.08，P=0.035）。与（GT+TT）基因型相比，GG基因型也显示出与GC风险增加有统计学关联（校正OR=1.38，95%CI：1.01-1.89，P=0.044）。在男性亚组中也观察到了这种情况（GG与TT：校正OR=1.71，95%CI：1.12-2.62，P=0.013；G等位基因与T等位基因：校正OR=1.32，95%CI：1.07-1.62，P=0.009）。幽门螺杆菌感染、吸烟和饮酒倾向于增强遗传因素对GC风险增加的影响，但其对GC风险的交互作用未达到统计学意义。
ERCC6 rs1917799 GG基因型可能与中国人尤其是男性的GC风险增加有关。
