College of Food Science, Northwest A&F University, Yangling 712100, People's Republic of China.
J Food Prot. 2013 Dec;76(12):2040-4. doi: 10.4315/0362-028X.JFP-13-224.
Prevalence of extended-spectrum β-lactamase (ESBL)-producing Salmonella in food is not well documented. This study investigated the prevalence of ESBL-producing Salmonella in 699 Salmonella isolates recovered from 1,152 retail chickens collected from six provinces and two national cities in the People's Republic of China in 2011. ESBL-producing isolates were screened by double-disk synergy test and confirmed using PCR and DNA sequencing. Of the 699 isolates tested, 60 (8.58%) were identified to be ESBL-producing Salmonella. Prevalence of ESBL-producing Salmonella was the highest in Shanghai city (17 [24.64%] of 69), followed by Shaanxi (10 [15.38%] of 65), Fujian (9 [11.69%] of 77), Guangdong (9 [7.69%] of 117), Sichuan (5 [7.25%] of 69), Beijing (6 [5.17%] of 116), Henan (4 [4.65%] of 86), and Guangxi (0 [0%] of 100) province. Significant difference (P < 0.05) in the prevalence of ESBL-producing Salmonella was found among six provinces and two cities. No significant difference (P > 0.05) in the prevalence was found between wet markets and supermarkets or between whole chickens and chopped chickens. The prevalence of ESBL-producing Salmonella differed significantly (P < 0.05) among different seasons, being higher in autumn than in spring and winter. Overall, ESBL-producing Salmonella varied significantly (P < 0.05) among 12 detected Salmonella serotypes: Abony (1 [33.33%] of 3), Indiana (28 [28.57%] of 98), Edinburg (6 [24.00%] of 25), Shubra (2 [20.00%] of 10), Uppsala (1 [16.67%] of 6), Thompson (8 [14.81%] of 54), Haardt (1 [12.50%] of 8), Agona (3 [9.68%] of 31), Gueuletapee (1 [6.25%] of 16), Typhimurium (4 [5.56%] of 72), Heidelberg (1 [4.55%] of 22), and Enteritidis (4 [3.17%] of 126). This study revealed that ESBL-producing Salmonella do exist in retail chicken in the People's Republic of China and that the potential risk of their presence in foods needs further exploration.
产超广谱β-内酰胺酶(ESBL)的沙门氏菌在食品中的流行情况尚未得到很好的记录。本研究调查了 2011 年从中国六个省和两个直辖市的 1152 只零售鸡中采集的 699 株沙门氏菌分离株中 ESBL 产生产生沙门氏菌的流行情况。通过双碟协同试验筛选产 ESBL 的分离株,并使用 PCR 和 DNA 测序进行确认。在测试的 699 株分离物中,有 60 株(8.58%)被鉴定为产 ESBL 的沙门氏菌。产 ESBL 的沙门氏菌的流行率在上海市最高(69 株中的 17 株[24.64%]),其次是陕西省(65 株中的 10 株[15.38%]),福建省(77 株中的 9 株[11.69%]),广东省(117 株中的 9 株[7.69%]),四川省(69 株中的 5 株[7.25%]),北京市(116 株中的 6 株[5.17%]),河南省(86 株中的 4 株[4.65%])和广西壮族自治区(100 株中的 0 株[0%])。六个省和两个城市之间产 ESBL 的沙门氏菌的流行率存在显着差异(P <0.05)。在湿市场和超市之间或整鸡和碎鸡之间,产 ESBL 的沙门氏菌的流行率没有显着差异(P > 0.05)。产 ESBL 的沙门氏菌的流行率在不同季节之间存在显着差异(P <0.05),秋季高于春季和冬季。总体而言,12 种检测到的沙门氏菌血清型之间产 ESBL 的沙门氏菌差异显着(P <0.05):Abony(3 株中的 1 株[33.33%]),Indiana(98 株中的 28 株[28.57%]),Edinburg(25 株中的 6 株[24.00%]),Shubra(10 株中的 2 株[20.00%]),Uppsala(6 株中的 1 株[16.67%]),Thompson(54 株中的 8 株[14.81%]),Haardt(8 株中的 1 株[12.50%]),Agona(31 株中的 3 株[9.68%]),Gueuletapee(16 株中的 1 株[6.25%]),Typhimurium(72 株中的 4 株[5.56%]),Heidelberg(22 株中的 1 株[4.55%])和Enteritidis(126 株中的 4 株[3.17%])。本研究表明,产 ESBL 的沙门氏菌确实存在于中国零售鸡中,其在食品中存在的潜在风险需要进一步探讨。