Shrestha Anil, Bajracharya Anup Muni, Subedi Hemraj, Turha Raju Shah, Kafle Sachin, Sharma Saroj, Neupane Sunil, Chaudhary Dhiraj Kumar
Department of Microbiology, Balkumari College, Chitwan, Nepal.
Department of Microbiology, Prithu Technical College, Institute of Agriculture and Animal Science, Tribhuvan University, Dang, Nepal.
BMC Res Notes. 2017 Nov 7;10(1):574. doi: 10.1186/s13104-017-2917-x.
Multidrug resistance (MDR) and extended spectrum beta lactamase (ESBL) producer Gram negative bacteria are considered as a major health problem, globally. ESBL enzyme hydrolyses the beta lactam ring of third generation cephalosporins, which alters the structure of the antibiotic. Due to the modification in structure of the antibiotic, bacteria show resistance to these antibiotics. Resistant bacterial strains are transmitted to humans from animals through consumption of uncooked meat, through contact with uncooked meat and meat surfaces. This study aims to assess bacteriological profile and analyze the situation of antibiotic resistance, multidrug resistance, and ESBL producing Gram negative bacteria in chicken meat.
A total of 38 chicken meat samples were studied in which 103 Gram negative bacteria were isolated. Species of Gram negative bacteria were identified as Citrobacter spp. (44.7%), Salmonella spp. (26.2%), Proteus spp. (18.4%), Escherichia coli (4.8%), Shigella spp. (3.9%), Pseudomonas spp. (1.9%), and Klebsiella spp. (1.0%). The prevalence of MDR isolates was found to be 79.6%. Total ESBL producer was 36.9% and ESBL producer among MDR was 34.9%. This concludes wide range of antibiotic resistance bacteria is prevalent in raw chicken meat.
耐多药（MDR）和产超广谱β-内酰胺酶（ESBL）的革兰氏阴性菌被认为是全球主要的健康问题。ESBL酶可水解第三代头孢菌素的β-内酰胺环，从而改变抗生素的结构。由于抗生素结构的改变，细菌对这些抗生素表现出耐药性。耐药菌株通过食用未煮熟的肉类、接触未煮熟的肉类和肉类表面从动物传播给人类。本研究旨在评估鸡肉中的细菌学特征，并分析产ESBL的革兰氏阴性菌的抗生素耐药性、耐多药情况。
共研究了38份鸡肉样本，从中分离出103株革兰氏阴性菌。革兰氏阴性菌的种类被鉴定为柠檬酸杆菌属（44.7%）、沙门氏菌属（26.2%）、变形杆菌属（18.4%）、大肠杆菌（4.8%）、志贺氏菌属（3.9%）、假单胞菌属（1.9%）和克雷伯氏菌属（1.0%）。耐多药分离株的流行率为79.6%。产ESBL的细菌总数为36.9%，耐多药菌株中产ESBL的细菌为34.9%。这表明生鸡肉中普遍存在多种抗生素耐药菌。
