Su Jin-Hui, Zhu Yao-Hong, Ren Tian-Yi, Guo Liang, Yang Gui-Yan, Jiao Lian-Guo, Wang Jiu-Feng
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Microorganisms. 2018 Nov 26;6(4):117. doi: 10.3390/microorganisms6040117.
can cause enteric diseases in humans and a wide range of animals, and even outbreaks of foodborne illness. The aim of this study was to investigate the frequency and distribution of serovars, and antimicrobial resistance in isolates from pigs with diarrhea in 26 provinces in China from 2014 to 2016. A total of 104 isolates were identified and the dominant serovar was 4,[5],12:i:- (53.9%). All isolates were resistant to trimethoprim-sulfamethoxazole, and many were resistant to ampicillin (80.8%) and tetracycline (76.9%). Among 104 isolates, was the dominant plasmid-mediated quinolone resistance gene (80.8%), followed by (47.1%). The pulsed-field gel electrophoresis results suggest that the isolates from different regions were genetically diverse, and ST34 was the most prevalent. 4,[5],12:i:- isolates is the widespread presence of heavy metal tolerance genes. The fact that the same sequence types were found in different regions and the high similarity coefficient of 4,[5],12:i:- isolates from different regions indicate the clonal expansion of the isolates, and the isolates carried various antimicrobial resistance genes. The multidrug resistant can be widely detected in pigs, which will present a challenge for farm husbandry.
可导致人类和多种动物患肠道疾病，甚至引发食源性疾病暴发。本研究旨在调查2014年至2016年中国26个省份腹泻猪分离株中血清型的频率和分布以及抗菌药物耐药性。共鉴定出104株分离株，优势血清型为4,[5],12:i: -（53.9%）。所有分离株对甲氧苄啶-磺胺甲恶唑耐药，许多对氨苄西林（80.8%）和四环素（76.9%）耐药。在104株分离株中，qnrB是主要的质粒介导喹诺酮耐药基因（80.8%），其次是qnrS（47.1%）。脉冲场凝胶电泳结果表明，不同地区的分离株在基因上具有多样性，ST34最为普遍。4,[5],12:i: -分离株中广泛存在重金属耐受基因。在不同地区发现相同的序列类型以及不同地区4,[5],12:i: -分离株的高相似系数表明分离株的克隆扩增，且分离株携带多种抗菌药物耐药基因。多重耐药的[具体细菌名称未给出]可在猪中广泛检测到，这将给养猪业带来挑战。
