Garland Shaun P, Wang Royal Y, Raghunathan Vijay Krishna, Lam Kit S, Murphy Christopher J, Russell Paul, Sun Gang, Pan Tingrui
Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California Davis, Davis, CA 95616, USA.
Department of Surgical & Radiological Sciences, School of Veterinary Medicine, University of California Davis, Davis, CA 95616, USA.
Biomaterials. 2014 Feb;35(5):1762-70. doi: 10.1016/j.biomaterials.2013.11.030. Epub 2013 Nov 28.
Oxidative stress, largely mediated by reactive oxygen species (ROS), is a nearly ubiquitous component in complex biological processes such as aging and disease. Optimal in vitro methods used in elucidating disease mechanisms would deliver of low levels of hydrogen peroxide, emulating the in vivo pathological state, but current methods are limited by kinetic stability or accurate measurement of the dose administered. Here we present an in vitro platform that exploits anthraquinone catalysts for the photocatalytic production of hydrogen peroxide. This system can be dynamically tuned to provide constant generation of hydrogen peroxide at a desired physiologic rate over at least 14 days and is described using a kinetic model. Material characterization and stability is discussed along with a proof-of-concept in vitro study that assessed the viability of cells as they were oxidatively challenged over 24 h at different ROS generation rates.
氧化应激主要由活性氧（ROS）介导，是衰老和疾病等复杂生物过程中几乎普遍存在的一个组成部分。用于阐明疾病机制的最佳体外方法应能产生低水平的过氧化氢，模拟体内病理状态，但目前的方法受到动力学稳定性或所给药剂量准确测量的限制。在此，我们展示了一个利用蒽醌催化剂光催化产生过氧化氢的体外平台。该系统可以动态调节，以至少14天的时间以所需的生理速率持续产生过氧化氢，并使用动力学模型进行了描述。同时讨论了材料表征和稳定性，以及一项概念验证体外研究，该研究评估了细胞在不同ROS生成速率下经过24小时氧化应激挑战后的活力。
