State Key Laboratory of Agrobiotechnology, MOA Key Laboratory of Soil Microbiology and National Energy R & D Center for Non-food Biomass, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
State Key Laboratory of Agrobiotechnology, MOA Key Laboratory of Soil Microbiology and National Energy R & D Center for Non-food Biomass, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Bioresour Technol. 2014;152:177-84. doi: 10.1016/j.biortech.2013.10.084. Epub 2013 Nov 5.
The effects of a variety of environmental factors on lipid accumulation of Chlorella minutissima UTEX 2341 under low-nutrition conditions were investigated. The growth of UTEX 2341 reached the exponential phase on the 1st day in low-nutrition medium. And the highest biomass productivity of 3.07 g L(-1) d(-1) was achieved on the 2nd day. The optimum pH and temperature for lipid accumulation were 6 and 20 °C respectively. 43.69 g L(-1) of NaCl and 0.11 mmol L(-1) of Fe(III) resulted in higher lipid content, analyzed by Design-Expert. And then under nitrogen starvation stress, the lipid content reached 22.84%. Meanwhile, the lipid yield was 2.5 g L(-1). The yield coefficient against carbon was 0.36 g g(-1), which was 4.68 times as much as that in OM medium. With the high proportion of C16 and C18 in the lipids, C. minutissima UTEX 2341 was proved to be a potential option for renewable biodiesel production.
研究了各种环境因素对低营养条件下小球藻 UTEX 2341 脂类积累的影响。在低营养培养基中，UTEX 2341 的生长在第 1 天达到指数期。第 2 天，生物量生产力达到最高，为 3.07 g L(-1) d(-1)。最适 pH 和温度分别为 6 和 20°C。设计专家分析得出，43.69 g L(-1)的 NaCl 和 0.11 mmol L(-1)的 Fe(III)可使脂含量更高。然后在氮饥饿胁迫下，脂含量达到 22.84%。同时，产率为 2.5 g L(-1)。碳对产率系数为 0.36 g g(-1)，是 OM 培养基中的 4.68 倍。由于 C16 和 C18 在脂质中的比例较高，因此小球藻 UTEX 2341 被证明是可再生生物柴油生产的一个潜在选择。
