在混合营养条件下培养新分离的微藻时，碳源对其生长和脂类积累的影响。
Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition.
机构信息
Department of Bioindustry Technology, Da-Yeh University, No. 168, University Rd., Dacun, Changhua 51591, Taiwan.
Department of Bioindustry Technology, Da-Yeh University, No. 168, University Rd., Dacun, Changhua 51591, Taiwan.
出版信息
Bioresour Technol. 2015 May;184:100-107. doi: 10.1016/j.biortech.2014.11.005. Epub 2014 Nov 18.
In order to produce microalgal lipids that can be transformed to biodiesel fuel, one isolate with high lipid content was identified as Chlorella sp. Y8-1. The growth and lipid productivity of an isolated microalga Chlorella sp. Y8-1 were investigated under different cultivation conditions, including autotrophic growth (CO2, with light), heterotrophic growth (sucrose, without light) and mixotrophic growth (organic carbon sources and CO2, with light). Mixotrophic Chlorella sp. Y8-1 showed higher lipid content (35.5±4.2%) and higher lipid productivity (0.01 g/L/d) than Chlorella sp. Y8-1 cultivated under autotrophic and heterotrophic conditions on modified Walne medium. Fatty acid analysis of Chlorella sp. Y8-1 showed the major presence of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acids (C18:3). The main fatty acid compositions of the Chlorella sp. Y8-1 are appropriate for biodiesel production.
为了生产可转化为生物柴油燃料的微藻油脂，鉴定出一株具有高油脂含量的藻株为小球藻 Y8-1。研究了分离的小球藻 Y8-1 在不同培养条件下的生长和油脂生产力，包括自养生长（CO2，光照）、异养生长（蔗糖，无光）和混合营养生长（有机碳源和 CO2，光照）。混合营养小球藻 Y8-1 表现出比小球藻 Y8-1 在改良 Walne 培养基上进行自养和异养培养更高的油脂含量（35.5±4.2%）和更高的油脂生产力（0.01 g/L/d）。小球藻 Y8-1 的脂肪酸分析表明，棕榈酸（C16:0）、油酸（C18:1）、亚油酸（C18:2）和亚麻酸（C18:3）的存在为主。小球藻 Y8-1 的主要脂肪酸组成适合生物柴油生产。
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