National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
Reprod Toxicol. 2014 Jan;43:85-93. doi: 10.1016/j.reprotox.2013.11.005. Epub 2013 Nov 27.
The deleterious damage of reproductive function following radiotherapy is of increasing importance. In the present study, we investigated the impact of long-term sodium selenite (SS) treatment on radiotherapy-induced ovarian injury in a rat model. Two-week after radiation exposure vaginal cyclicity was arrested, and serum FSH level was elevated in irradiated female rats. SS significantly ameliorated ovarian and uterine oxidative stress induced by irradiation through decreasing the lipid peroxide level and increasing the glutathione level, and glutathione peroxidase activity. In the presence of SS, ovarian cytochrome c and caspase 3 expressions triggered by radiotherapy were decreased. SS significantly counteracted radiation-induced a widespread loss of ovarian follicles and caused further stimulation of follicular proliferation through enhancing PCNA expression. Despite such alteration in ovarian function, serum estradiol level did not change after irradiation, whereas SS significantly increased it. In conclusion, long-term SS treatment improved reproductive development, which was impaired by radiotherapy.
放疗后生殖功能的有害损害变得越来越重要。在本研究中，我们研究了长期亚硒酸钠（SS）治疗对大鼠模型中放疗诱导的卵巢损伤的影响。照射后两周，阴道周期性被阻断，照射雌性大鼠的血清 FSH 水平升高。SS 通过降低脂质过氧化物水平和增加谷胱甘肽水平以及谷胱甘肽过氧化物酶活性，显著改善了照射引起的卵巢和子宫氧化应激。在 SS 的存在下，放疗引发的卵巢细胞色素 c 和半胱天冬酶 3 的表达减少。SS 通过增强 PCNA 表达，显著对抗辐射引起的广泛卵巢卵泡丧失，并进一步刺激卵泡增殖。尽管卵巢功能发生了这种变化，但照射后血清雌二醇水平没有变化，而 SS 则显著增加了它。总之，长期 SS 治疗改善了受放疗损害的生殖发育。
