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载有人脂肪间充质干细胞的蛋白支架对大鼠放射性阴道损伤的修复作用。

Healing effects of a protein scaffold loaded with adipose-derived mesenchymal stem cells on radiation-induced vaginal injury in rats.

机构信息

Medical School of Chinese PLA, Department of Obstetrics and Gynecology The First Medical Center, Chinese PLA General Hospital, Beijing, China.

Department of Obstetrics and Gynecology, The First Medical Center of Chinese PLA General Hospital, Beijing, China.

出版信息

J Int Med Res. 2020 Oct;48(10):300060520958826. doi: 10.1177/0300060520958826.

DOI:10.1177/0300060520958826
PMID:33115306
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7607296/
Abstract

OBJECTIVES

Cervical cancer, the most common female cancer after breast cancer, is typically treated using radiotherapy. However, pelvic radiotherapy can cause irreversible damage to the vagina, seriously affecting patients' quality of life. In this study, protein scaffolds loaded with rat adipose-derived mesenchymal stem cells (ADSCs) were implanted into irradiated tissue to assess their healing potential.

METHODS

We established a rat model of radiation-induced vaginal injury. Complexes (consisting of protein scaffolds loaded with ADSCs) were implanted into injury sites. Histological analysis were used to assess regeneration of the vaginal epithelium. RNA sequencing was used to study the therapeutic mechanism of the complexes.

RESULTS

The complexes promoted vaginal epithelial cell regeneration, vaginal tissue repair and improved vaginal stenosis and contracture. Compared with rats transplanted with ADSCs, rats transplanted with complexes achieved better therapeutic effects.

CONCLUSIONS

Protein scaffold-ADSC complexes had a beneficial therapeutic effect on radiation-induced vaginal injury in rats and may serve as the basis of a novel therapeutic approach for radiation dermatitis.

摘要

目的

宫颈癌是继乳腺癌之后女性最常见的癌症，通常采用放射治疗。然而，盆腔放疗会对阴道造成不可逆转的损伤，严重影响患者的生活质量。本研究将负载大鼠脂肪间充质干细胞（ADSCs）的蛋白支架植入到照射组织中，评估其愈合潜能。

方法

我们建立了大鼠放射性阴道损伤模型。将复合物（由负载 ADSC 的蛋白支架组成）植入到损伤部位。组织学分析用于评估阴道上皮的再生情况。RNA 测序用于研究复合物的治疗机制。

结果

复合物促进了阴道上皮细胞的再生、阴道组织的修复，改善了阴道狭窄和挛缩。与移植 ADSC 的大鼠相比，移植复合物的大鼠取得了更好的治疗效果。

结论

蛋白支架-ADSC 复合物对大鼠放射性阴道损伤具有有益的治疗作用，可能为放射性皮炎的一种新的治疗方法提供依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/5a1dc03b824f/10.1177_0300060520958826-fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/f25514f6a53c/10.1177_0300060520958826-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/378186992dcc/10.1177_0300060520958826-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/421211fd0b55/10.1177_0300060520958826-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/1eaf6e6f6e0b/10.1177_0300060520958826-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/e34683941949/10.1177_0300060520958826-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/b82812cd9287/10.1177_0300060520958826-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/24abb303bda3/10.1177_0300060520958826-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/b4541b83dc3a/10.1177_0300060520958826-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/117c2f3e6356/10.1177_0300060520958826-fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/5a1dc03b824f/10.1177_0300060520958826-fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/f25514f6a53c/10.1177_0300060520958826-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/378186992dcc/10.1177_0300060520958826-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/421211fd0b55/10.1177_0300060520958826-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/1eaf6e6f6e0b/10.1177_0300060520958826-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/e34683941949/10.1177_0300060520958826-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/b82812cd9287/10.1177_0300060520958826-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/24abb303bda3/10.1177_0300060520958826-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/b4541b83dc3a/10.1177_0300060520958826-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/117c2f3e6356/10.1177_0300060520958826-fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddbd/7607296/5a1dc03b824f/10.1177_0300060520958826-fig10.jpg

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