Whitaker J N, Gupta M, Smith O F
Ann Neurol. 1986 Sep;20(3):329-36. doi: 10.1002/ana.410200310.
To define in more detail the features of the immunoreactive myelin basic protein (MBP) present in cerebrospinal fluid (CSF) of humans following acute injury to central nervous system myelin, the epitopes of MBP recognized by three different antisera, each capable of detecting immunoreactive MBP in CSF, were examined. All three antisera reacted well with human MBP and human MBP peptide 45-89. Only in radioimmunoassays in which the MBP peptide 45-89 served as the radioligand could clearly elevated values of immunoreactive MBP be measured in CSF specimens from 5 patients with multiple sclerosis during or immediately after an exacerbation. The two antisera that reacted well with MBP peptide 80-89 resulted in higher levels of immunoreactive MBP measured in CSF. An epitope present in human MBP peptide 80-89 but sharing a conformation with both MBP and MBP peptide 45-89 is present in CSF following acute central nervous system myelin damage in multiple sclerosis.
为了更详细地界定急性中枢神经系统髓鞘损伤后人类脑脊液（CSF）中免疫反应性髓鞘碱性蛋白（MBP）的特征，我们检测了三种不同抗血清所识别的MBP表位，每种抗血清都能够检测CSF中的免疫反应性MBP。所有三种抗血清与人MBP和人MBP肽45 - 89反应良好。仅在以MBP肽45 - 89作为放射性配体的放射免疫分析中，能够在5例多发性硬化症患者病情加重期间或加重后立即采集的CSF标本中检测到明显升高的免疫反应性MBP值。与MBP肽80 - 89反应良好的两种抗血清导致在CSF中测得的免疫反应性MBP水平更高。在多发性硬化症急性中枢神经系统髓鞘损伤后，CSF中存在一种存在于人MBP肽80 - 89中但与MBP和MBP肽45 - 89具有相同构象的表位。
