Chung Yeon Soo, Kwon Ka Hee, Shin Sook, Kim Jae Hong, Park Yong Ho, Yoon Jang Won
College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea.
J Microbiol Biotechnol. 2014 Mar 28;24(3):386-93. doi: 10.4014/jmb.1310.10088.
Possible cross-transmission of hospital-associated enterococci between human patients, medical staff, and hospital environments has been extensively studied. However, limited information is available for veterinary hospital-associated Enterococcus isolates. This study investigated the possibility of cross-transmission of antibiotic-resistant enterococci between dog patients, their owners, veterinary staff, and hospital environments. Swab samples (n =46 5) were obtained from five veterinary hospitals in Seoul, Korea, during 2011. Forty-three Enterococcus strains were isolated, representing seven enterococcal species. E. faecalis and E. faecium were the most dominant species (16 isolates each, 37.2%). Although slight differences in the antibiotic resistance profiles were observed between the phenotypic and the genotypic data, our antibiogram analysis demonstrated high prevalence of the multiple drug-resistant (MDR) isolates of E. faecalis (10/16 isolates, 62.5%) and E. faecium (12/16 isolates, 75.0%). Pulsed-field gel electrophoretic comparison of the MDR isolates revealed three different clonal sets of E. faecalis and a single set of E. faecium, which were isolated from different sample groups or dog patients at the same or two separate veterinary hospitals. These results imply a strong possibility of cross-transmission of the antibiotic-resistant enterococcal species between animal patients, owners, veterinary staff, and hospital environments.
医院相关肠球菌在人类患者、医护人员和医院环境之间可能的交叉传播已得到广泛研究。然而，关于兽医医院相关肠球菌分离株的信息有限。本研究调查了耐抗生素肠球菌在犬类患者、其主人、兽医工作人员和医院环境之间交叉传播的可能性。2011年期间，从韩国首尔的五家兽医医院采集了拭子样本（n = 465）。分离出43株肠球菌菌株，代表7种肠球菌。粪肠球菌和屎肠球菌是最主要的菌种（各16株，占37.2%）。虽然在表型和基因型数据之间观察到抗生素耐药谱存在细微差异，但我们的抗菌谱分析表明，粪肠球菌（10/16株，62.5%）和屎肠球菌（12/16株，75.0%）的多重耐药（MDR）分离株患病率很高。对MDR分离株进行脉冲场凝胶电泳比较，发现粪肠球菌有三种不同的克隆组，屎肠球菌有一组，这些分离株来自同一或两家不同兽医医院的不同样本组或犬类患者。这些结果表明，耐抗生素肠球菌在动物患者、主人、兽医工作人员和医院环境之间有很强的交叉传播可能性。
