Department of BIN Fusion Technology, Polymer Fusion Research Center, Department of Polymer Nano Science Technology, Chonbuk National University, Jeonju.
Int J Stem Cells. 2011 Nov;4(2):105-12. doi: 10.15283/ijsc.2011.4.2.105.
Alginate, an anionic polysaccharide distributed widely in the cell walls of brown algae, is used in biomedical applications. However, alginate' s performance as a biomaterial, has limited by its several contamination such as endotoxins, proteins and polyphenols.
To overcome this problem, we have developed using modified Korbutt method for alginate purification. After purification, we made alginate films and used for retinal pigment epithelial cell (RPEs) regeneration. ARPE-19 cells were seeded in non-purified and purified alginate films, and then cell viability and proliferation were estimated by MTT assay and RT-PCR was performed to assess specific cell expression. ARPE-19 cell-loaded alginate films were evaluated specific protein expression by through AEC staining and we examined the cell adhesion by scanning electron micro scopy (SEM).
In this result, ARPE-19 cells in purified alginate films had higher cell proliferative rate and phenotypic expression than those on non-purified alginate films. The results suggest that purified alginate is useful for RPEs regeneration.
褐藻细胞壁中广泛分布的阴离子多糖——藻酸盐，在生物医学领域得到了广泛的应用。然而，藻酸盐作为生物材料的性能受到其几种污染物的限制，如内毒素、蛋白质和多酚。
为了解决这个问题，我们采用改良的科巴特法对藻酸盐进行了纯化。纯化后，我们制备了藻酸盐薄膜，并用于视网膜色素上皮细胞（RPE）的再生。将 ARPE-19 细胞接种在未纯化和纯化的藻酸盐薄膜上，然后通过 MTT 法评估细胞活力和增殖，并通过 RT-PCR 评估特定细胞的表达。通过 AEC 染色评估载有 ARPE-19 细胞的藻酸盐薄膜的特定蛋白表达，并通过扫描电子显微镜（SEM）检查细胞黏附情况。
在本研究中，与非纯化藻酸盐薄膜相比，纯化藻酸盐薄膜中的 ARPE-19 细胞具有更高的细胞增殖率和表型表达。这些结果表明，纯化藻酸盐可用于 RPE 再生。
