Histology Department, Faculty of Medicine, Cairo University.
Int J Stem Cells. 2012 Nov;5(2):130-9. doi: 10.15283/ijsc.2012.5.2.130.
It was postulated that adriamycin (ADR) induce renal tubulointerstitial injury. Clinicians are faced with a challenge in producing response in renal patients and slowing or halting the evolution towards kidney failure. The present study aimed at investigating the relation between the possible therapeutic effect of human mesenchymal stem cells (HMSCs), isolated from cord blood on tubular renal damage and their distribution by using ADR induced nephrotoxicity as a model in albino rat.
Thirty three male albino rats were divided into control group, ADR group where rats were given single intraperitoneal (IP) injection of 5 mg/kg adriamycin. The rats were sacrificed 10, 20 and 30 days following confirmation of tubular injury. In stem cell therapy group, rats were injected with HMSCs following confirmation of renal injury and sacrificed 10, 20 and 30 days after HMSCs therapy. Kidney sections were exposed to histological, histochemical, immunohistochemical, morphometric and serological studies. In response to SC therapy, vacuolated cytoplasm, dark nuclei, detached epithelial lining and desquamated nuclei were noticed in few collecting tubules (CT). 10, 20 and 30 days following therapy. The mean count of CT showing desquamated nuclei and mean value of serum creatinine revealed significant difference in ADR group. The mean area% of Prussian blue+ve cells and that of CD105 +ve cells measured in subgroup S1 denoted a significant increase compared to subgroups S2 and S3.
ADR induced tubulointerstitial damage that regressed in response to cord blood HMSC therapy.
阿霉素（ADR）被认为会导致肾小管间质损伤。临床医生面临的挑战是如何在肾病患者中产生疗效，以及减缓或阻止向肾衰竭的发展。本研究旨在探讨人脐带血间充质干细胞（HMSCs）的可能治疗效果与肾小管损伤之间的关系，并通过使用 ADR 诱导的肾毒性作为白化大鼠模型来研究其分布。
33 只雄性白化大鼠被分为对照组、ADR 组，ADR 组大鼠腹腔内单次注射 5mg/kg 阿霉素。在确认肾小管损伤后第 10、20 和 30 天处死大鼠。在干细胞治疗组，在确认肾损伤后给予 HMSCs 注射，并在 HMSCs 治疗后第 10、20 和 30 天处死大鼠。对肾组织切片进行组织学、组织化学、免疫组织化学、形态计量学和血清学研究。在接受 SC 治疗后，少数集合管（CT）中可见空泡化细胞质、深染核、脱落的上皮衬里和脱核。治疗后 10、20 和 30 天。显示脱核的 CT 的平均计数和血清肌酐的平均值在 ADR 组中差异显著。在亚组 S1 中测量的普鲁士蓝+ve 细胞和 CD105+ve 细胞的平均面积%与亚组 S2 和 S3 相比显著增加。
ADR 诱导的肾小管间质损伤在脐带血 HMSC 治疗后消退。
