Poosala Pornthida, Kitaoka Takuya
Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Biomolecules. 2016 Jan 15;6(1):12. doi: 10.3390/biom6010012.
Skeletal muscle possesses a robust capacity to regenerate functional architectures with a unidirectional orientation. In this study, we successfully arranged skeletal myoblast (C2C12) cells along micropatterned gold strips on which chitohexaose was deposited via a vectorial chain immobilization approach. Hexa-N-acetyl-D-glucosamine (GlcNAc6) was site-selectively modified at its reducing end with thiosemicarbazide, then immobilized on a gold substrate in striped micropatterns via S-Au chemisorption. Gold micropatterns ranged from 100 to 1000 µm in width. Effects of patterning geometries on C2C12 cell alignment, morphology, and gene expression were investigated. Unidirectional alignment of C2C12 cells having GlcNAc6 receptors was clearly observed along the micropatterns. Decreasing striped pattern width increased cell attachment and proliferation, suggesting that the fixed GlcNAc6 and micropatterns impacted cell function. Possibly, interactions between nonreducing end groups of fixed GlcNAc6 and cell surface receptors initiated cellular alignment. Our technique for mimicking native tissue organization should advance applications in tissue engineering.
骨骼肌具有强大的能力来再生具有单向取向的功能结构。在本研究中，我们成功地将骨骼肌成肌细胞（C2C12）沿微图案化的金带排列，通过矢量链固定方法在金带上沉积了壳六糖。六 - N - 乙酰 - D - 葡萄糖胺（GlcNAc6）在其还原端用氨基硫脲进行位点选择性修饰，然后通过S - Au化学吸附以条纹微图案形式固定在金基底上。金微图案的宽度范围为100至1000μm。研究了图案几何形状对C2C12细胞排列、形态和基因表达的影响。沿着微图案清晰地观察到具有GlcNAc6受体的C2C12细胞的单向排列。条纹图案宽度的减小增加了细胞附着和增殖，表明固定的GlcNAc6和微图案影响了细胞功能。可能是固定的GlcNAc6的非还原端基团与细胞表面受体之间的相互作用引发了细胞排列。我们模拟天然组织结构的技术应能推动组织工程中的应用。
