Bio-Nano Electronics Research Centre, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan.
Carbohydr Polym. 2013 Feb 15;92(2):1225-33. doi: 10.1016/j.carbpol.2012.10.033. Epub 2012 Oct 22.
Extremophilic bacterial polysaccharide based biocompatible nanofibers were produced for the first time via electrospinning technique. Mauran (MR), an extremophilic sulfated exopolysaccharide was extracted from moderately halophilic bacterium, Halomonas maura and characterized for the application of nanofiber synthesis. Thin-uniform MR nanofibers were produced using homogenous solutions of poly (vinyl alcohol) (PVA) blended with different concentrations of MR. Characterization of complex MR/PVA nanofibers were performed using scanning electron microscope and analyzed for the cytotoxicity using mouse fibroblast cells as well as mesenchymal stem cells. An average of 120 nm sized nanofibers were produced and tested for an enhanced cell growth under in vitro conditions in comparison with control. MR and MR/PVA nanofibers were found to be an excellent biomaterial for the migration, proliferation and differentiation of mammalian cells, which was confirmed by cell adhesion studies and confocal microcopy. Interestingly, biological and physicochemical properties of MR hasten the application of MR based nanofibers for various biomedical applications like tissue engineering and drug delivery.
首次通过静电纺丝技术生产了基于极端细菌多糖的生物相容性纳米纤维。从中度嗜盐细菌盐单胞菌中提取了极端嗜盐的硫酸化胞外多糖 Mauran（MR），并对其用于纳米纤维合成进行了表征。使用聚（聚乙烯醇）（PVA）与不同浓度的 MR 均匀混合的均相溶液来生产薄而均匀的 MR 纳米纤维。使用扫描电子显微镜对复杂的 MR/PVA 纳米纤维进行了表征，并使用小鼠成纤维细胞和间充质干细胞进行了细胞毒性分析。平均产生 120nm 大小的纳米纤维，并在体外条件下进行了增强细胞生长的测试，与对照相比。MR 和 MR/PVA 纳米纤维被证明是一种优异的生物材料，可促进哺乳动物细胞的迁移、增殖和分化，这通过细胞黏附研究和共聚焦显微镜得到了证实。有趣的是，MR 的生物学和物理化学性质加速了基于 MR 的纳米纤维在组织工程和药物输送等各种生物医学应用中的应用。
