State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
Carbohydr Polym. 2014 Jan 30;101:975-81. doi: 10.1016/j.carbpol.2013.10.045. Epub 2013 Oct 21.
A new strain SK 21.002 which could produce intracellular levansucrase was isolated from beet sugar growing soil. It was identified as Bacillus methylotrophicus based on the sequence analysis of the 16S rRNA and the morphological characteristic. The structure of levan biosynthesized from the levansucrase was determined to be connected through β-(2→6) linkages with fructofuranosyl residues. The molecular weight of the product was between 4 and 5 kDa. The optimal conditions to produce levan using the levansucrase were studied. The presence of Mg(2+) could increase the yield of levan. The yield of levan reached about 100g/L with the enzyme under the optimum conditions.
从甜菜糖种植土壤中分离到一株能够产生胞内蔗聚糖酶的新型 SK 21.002 菌株。根据 16S rRNA 序列分析和形态学特征，将其鉴定为甲基营养芽孢杆菌。从该蔗聚糖酶合成的蔗聚糖的结构确定为通过β-（2→6）键与果糖呋喃糖残基连接。产物的分子量在 4 到 5 kDa 之间。研究了使用该蔗聚糖酶生产蔗聚糖的最佳条件。Mg(2+) 的存在可以提高蔗聚糖的产量。在最佳条件下，该酶的蔗聚糖产量约为 100g/L。
