豌豆（Pisum sativum）染色质的核小体重复长度在发芽过程中发生变化。

The nucleosomal repeat length of pea (Pisum sativum) chromatin changes during germination.

机构信息

Department of Biochemistry, Faculties of Sciences, University of Valencia, Burjassot, Valencia, Spain.

出版信息

Plant Mol Biol. 1986 Jan;7(1):25-31. doi: 10.1007/BF00020128.

DOI:10.1007/BF00020128

Abstract

Pea (Pisum sativum) nuclei have been isolated from ungerminated embryos, developing embryonic axes and seedlings. Morphological and biochemical criteria revealed that preparations were free from contaminants and that nuclei were intact. These circumstances permitted an accurate determination of nucleosomal repeat lengths, the values obtained being 175±4 base pairs for ungerminated embryos, 185±5 base pairs for 62-hours germinated embryonic axes and 185±3 base pairs for 6-day old seedlings. The results seem to indicate that the increase in repeat length is associated with the onset of transcription and/or replication of DNA.

摘要

豌豆核已从未发芽的胚胎、发育中的胚胎轴和幼苗中分离出来。形态学和生化标准表明，这些制备物没有受到污染，并且核是完整的。这些情况使得核小体重复长度的精确测定成为可能，得到的数值分别为未发芽胚胎 175±4 个碱基对、62 小时发芽胚胎轴 185±5 个碱基对和 6 天龄幼苗 185±3 个碱基对。结果似乎表明，重复长度的增加与 DNA 的转录和/或复制的开始有关。