Department of Life Science, Gachon University, Seongnam-Si, Kyeonggi-Do, Republic of Korea.
PLoS One. 2013 Nov 26;8(11):e82688. doi: 10.1371/journal.pone.0082688. eCollection 2013.
We have previously reported that seventy percent ethanol extract of Chrysanthemum indicum Linne (CIE) strongly reduces Epstein-Barr virus (EBV)-transformed lymphoblastoid cell line (LCL) survival by inhibiting virus-encoded latent infection membrane protein 1 (LMP1)-induced NF-κB activation. To identify an active compound(s) in CIE that inhibits LMP1-induced NF-κB activation, activity-guided fractionation was employed. The CH2Cl2 fraction of CIE strongly reduced LMP1-induced NF-κB activation and LCL viability with relatively low cytotoxic effects on primary human foreskin fibroblast (HFF), HeLa or Burkitt's lymphoma (BL41) cells. Furthermore, lupeol, a pentacyclic triterpene, was identified in the CH2Cl2 fraction of CIE to attenuate LMP1-induced NF-κB activation and LCL viability. This study demonstrates that lupeol is one of active compounds in the CH2Cl2 fraction of CIE that inhibits LMP1-induced NF-κB activation and reduces NF-κB-dependent LCL viability.
我们之前曾报道过，菊花（CIE）的 70%乙醇提取物通过抑制病毒编码的潜伏感染膜蛋白 1（LMP1）诱导的 NF-κB 激活，强烈降低 Epstein-Barr 病毒（EBV）转化的淋巴母细胞系（LCL）的存活率。为了鉴定 CIE 中抑制 LMP1 诱导的 NF-κB 激活的活性化合物（s），采用了活性导向的分级分离。CIE 的 CH2Cl2 馏分强烈降低了 LMP1 诱导的 NF-κB 激活和 LCL 的活力，对原代人包皮成纤维细胞（HFF）、HeLa 或 Burkitt 淋巴瘤（BL41）细胞的细胞毒性相对较低。此外，在 CIE 的 CH2Cl2 馏分中鉴定出了羽扇醇，一种五环三萜，可减弱 LMP1 诱导的 NF-κB 激活和 LCL 的活力。本研究表明，羽扇醇是 CIE 的 CH2Cl2 馏分中抑制 LMP1 诱导的 NF-κB 激活和降低 NF-κB 依赖性 LCL 活力的活性化合物之一。
