Facultad de Medicina, Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS), Universidad de Buenos Aires, Paraguay 2155, C1121ABG, Ciudad Autónoma de Buenos Aires, Argentina,
Arch Virol. 2014 May;159(5):1109-17. doi: 10.1007/s00705-013-1917-y. Epub 2013 Dec 4.
In Argentina, current procedures to ensure the safety of the blood supply for transfusion include the serologic detection of specific blood-borne infections. The aim of this study was to evaluate the prevalence and the genetic diversity of hepatitis B virus (HBV) and hepatitis D virus (HDV) in blood donor populations from two distantly located Argentine regions. Data from 56,983 blood donations from the Favaloro Foundation, in the city of Buenos Aires (Central Region), and the Central Blood Bank of Misiones Province (Northeast Region) were analyzed. Samples that were reactive for HBsAg were analyzed for HBV-DNA characterization and HDV serological and molecular analysis. The HBV prevalence was 0.12 % for HBsAg and 1.68 % for anti-HBc antibodies in Buenos Aires, and 0.73 % and 8.55 %, respectively, in Misiones. Seventy-seven HBsAg-reactive samples were analyzed by polymerase chain reaction for HBV-DNA. Subgenotypes A2, B2, C2, F1b and F4 (Buenos Aires) and F1b and D3 (Misiones) were detected. Several mutations within the major hydrophilic region of HBsAg, the reverse transcriptase, the basal core promoter, and the precore/core were detected. HDV genotype 1 was identified in Buenos Aires. This study confirms the circulation of several HBV subgenotypes, as well as known and newly identified variants, and the presence of HDV1 in this population. A thorough investigation has to be carried out to evaluate the clinical importance of some of the documented mutations as well as those detected in the HDV1 case.
在阿根廷，确保输血用血液供应安全的现行程序包括对特定血源感染进行血清学检测。本研究旨在评估来自阿根廷两个偏远地区的献血人群中乙型肝炎病毒（HBV）和丁型肝炎病毒（HDV）的流行率和遗传多样性。分析了来自布宜诺斯艾利斯市（中部地区）的法瓦罗基金会和米西奥内斯省中央血库（东北地区）的 56983 份献血样本的数据。对 HBsAg 反应性样本进行 HBV-DNA 特征分析和 HDV 血清学及分子分析。布宜诺斯艾利斯的 HBsAg 流行率为 0.12%，抗-HBc 抗体为 1.68%，米西奥内斯的分别为 0.73%和 8.55%。对 77 份 HBsAg 反应性样本进行聚合酶链反应以检测 HBV-DNA。检测到亚基因型 A2、B2、C2、F1b 和 F4（布宜诺斯艾利斯）和 F1b 和 D3（米西奥内斯）。在 HBsAg 主要亲水区、逆转录酶、基本核心启动子和前核心/核心内检测到多个突变。在布宜诺斯艾利斯鉴定出 HDV 基因型 1。本研究证实了几种 HBV 亚基因型的流行，以及已知和新发现的变异体，以及该人群中 HDV1 的存在。必须进行彻底调查，以评估一些已记录的突变以及在 HDV1 病例中检测到的突变的临床重要性。
