Ishiwata I, Ishiwata C, Soma M, Ono I, Nakaguchi T, Ishikawa H
Ishiwata Obstetrics and Gynecologic Hospital, Ibaraki-ken, Japan.
Gynecol Oncol. 1988 Jan;29(1):87-93. doi: 10.1016/0090-8258(88)90151-5.
Tumor angiogenic activity (TAA) from tumor angiogenesis factor (TAF), produced by 24 cell lines of various kinds of gynecologic tumors, was assayed onto chorioallantoic membranes (CAMs) of chick embryos. Methylcellulose (1%) pellets containing 1 x 10(7) cells were placed on 8-day-old postfertilized CAMs, and the grade of neovascularization was assayed 3 days after inoculation. Neovascularization occurred prominently in such cell lines, as HTBOA (poorly differentiated ovarian carcinoma), HUOCA-II (poorly differentiated clear cell adenocarcinoma), HWUA (poorly differentiated endometrial adenocarcinoma), and in HKUS (uterine cervical small cell carcinoma); however, neovascularization did not occur in SNK (uterine leiomyosarcoma line). The cell lines which secreted TAF showed high heterotransplantability in the nude mice and produced rapidly growing tumors which were rich in blood vessels. However, the SKN line which did not secret TAF was not transplantable. These results suggested that there was a close relationship among TAA, transplantability, and tumor growth rate.
对来源于各种妇科肿瘤的24种细胞系所产生的肿瘤血管生成因子（TAF）的肿瘤血管生成活性（TAA），在鸡胚绒毛尿囊膜（CAM）上进行测定。将含有1×10⁷个细胞的1%甲基纤维素小球置于受精后8天的CAM上，并在接种3天后测定血管生成等级。血管生成在如下细胞系中显著发生，如HTBOA（低分化卵巢癌）、HUOCA-II（低分化透明细胞腺癌）、HWUA（低分化子宫内膜腺癌）以及HKUS（子宫颈小细胞癌）；然而，在SNK（子宫平滑肌肉瘤细胞系）中未发生血管生成。分泌TAF的细胞系在裸鼠中显示出高异种移植性，并产生富含血管的快速生长肿瘤。然而，不分泌TAF的SKN细胞系不可移植。这些结果表明，TAA、移植性和肿瘤生长速率之间存在密切关系。
