Limberger R J, Charon N W
J Bacteriol. 1986 Nov;168(2):1030-2. doi: 10.1128/jb.168.2.1030-1032.1986.
"Treponema phagedenis" periplasmic flagella (PF) have two major protein bands at molecular weights of 33,000 and 39,800 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (R. J. Limberger and N. W. Charon, J. Bacteriol. 166:105-112, 1986). By use of Western blotting and a polyclonal antiserum directed toward the 33,000-molecular-weight PF protein, cell lysates of 12 species of spirochetes were surveyed for reactivity. Eight species of Treponema as well as Spirochaeta aurantia were positive. The results suggest that epitopes residing on the 33,000-molecular-weight PF protein of "T. phagedenis" are evolutionarily well conserved among the spirochetes.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定（R. J. Limberger和N. W. Charon，《细菌学杂志》166:105 - 112，1986年），“溃蚀性密螺旋体”的周质鞭毛（PF）有两条主要蛋白带，分子量分别为33,000和39,800。利用蛋白质印迹法和针对分子量为33,000的PF蛋白的多克隆抗血清，检测了12种螺旋体的细胞裂解物的反应性。8种密螺旋体以及橙色螺旋体呈阳性。结果表明，“溃蚀性密螺旋体”分子量为33,000的PF蛋白上的表位在螺旋体中进化上高度保守。
