Wos S M, Wicher K
Int Arch Allergy Appl Immunol. 1986;79(3):282-5. doi: 10.1159/000233987.
Extensive cross reactivity between Treponema pallidum and three nonpathogenic species of treponemes was demonstrated by the Western blot technique. Rabbit antiserum produced by adjuvant immunization with solubilized T. pallidum antigens reacted with 34 T. pallidum antigens and with approximately 30 antigens each of T. phagedenis biotype Reiter, T. noguchii and T. vincentii. Adsorption of the antiserum with T. phagedenis Reiter removed only about half of the cross-reacting antibodies. Sequential adsorption with all three nonpathogenic treponemes removed antibodies to all but three polypeptides of 36,000, 34,000 and 27,000 daltons.
通过蛋白质印迹技术证明了梅毒螺旋体与三种非致病性密螺旋体之间存在广泛的交叉反应。用溶解的梅毒螺旋体抗原进行佐剂免疫产生的兔抗血清与34种梅毒螺旋体抗原发生反应，并且与侵蚀艾肯菌生物型赖特尔、野口密螺旋体和奋森密螺旋体各自的约30种抗原发生反应。用侵蚀艾肯菌生物型赖特尔吸附抗血清仅去除了约一半的交叉反应抗体。用所有三种非致病性密螺旋体依次吸附，除了针对分子量为36,000、34,000和27,000道尔顿的三种多肽的抗体外，其余抗体均被去除。
