Luther B, Moskophidis M
Hygienisches Institut, Medizinaluntersuchungsanstalt, Hamburg.
Zentralbl Bakteriol. 1990 Nov;274(2):214-26. doi: 10.1016/s0934-8840(11)80104-0.
The antigenic cross-reactivity between different Borrelia and Treponema species was determined by the indirect immunofluorescence antibody test and sodium dodecyl sulphate-polyacrylamide gel electrophoresis followed by immunoblotting. The protein profiles of Borrelia burgdorferi, Borrelia recurrentis, Treponema pallidum, and Treponema phagedenis revealed essential differences. Using immunoblotting, rabbit immune sera to B. burgdorferi and B. recurrentis exhibited strong cross-reactivities to heterologous borrelial antigens and, to a lesser extent, to treponemal antigens. Immune sera to T. pallidum and T. phagedenis reacted with heterologous treponemal antigens, but exhibited lesser cross-reactivities to borrelial antigens. Five B. burgdorferi and seven T. pallidum major antigens were not cross-reacting with antisera raised against T. pallidum and B. burgdorferi, respectively. However, absorption of the investigated antisera with a T. phagedenis ultrasonicate eliminated cross-reacting borrelial and treponemal antibodies.
通过间接免疫荧光抗体试验以及十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳后免疫印迹法，测定了不同疏螺旋体属和密螺旋体属物种之间的抗原交叉反应性。伯氏疏螺旋体、回归热疏螺旋体、梅毒螺旋体和溃蚀性密螺旋体的蛋白质谱显示出本质差异。利用免疫印迹法，针对伯氏疏螺旋体和回归热疏螺旋体的兔免疫血清对异源疏螺旋体抗原表现出强烈的交叉反应，对密螺旋体抗原的交叉反应程度较小。针对梅毒螺旋体和溃蚀性密螺旋体的免疫血清与异源密螺旋体抗原发生反应，但对疏螺旋体抗原的交叉反应较小。分别有5种伯氏疏螺旋体主要抗原和7种梅毒螺旋体主要抗原不与针对梅毒螺旋体和伯氏疏螺旋体产生的抗血清发生交叉反应。然而，用溃蚀性密螺旋体超声裂解物吸收所研究的抗血清可消除交叉反应的疏螺旋体和密螺旋体抗体。
