Tyner A L, Fuchs E
J Cell Biol. 1986 Nov;103(5):1945-55. doi: 10.1083/jcb.103.5.1945.
Keratin K6 is a protein that is expressed in human skin under conditions of hyperproliferation (e.g., wound-healing, psoriasis, and cell culture) and malignant transformation (e.g., squamous cell carcinomas). When induced, the appearance of K6 is rapid: if skin tissue is placed in radiolabeled culture medium, this protein can be detected within an hour. The regulation of K6 seems to be controlled partly by a posttranscriptional mechanism: At least two K6 genes are actively transcribed both in vivo, when the protein is not made, as well as in vitro, when abundant levels of the protein are expressed. Substantial levels of K6a and K6b RNAs can be detected in skin by Northern Blot analysis, and these RNAs are largely, if not fully translatable in vitro. In situ hybridizations reveal that the RNAs are distributed throughout the living layers of the epidermis. The rapid induction of K6 expression through a posttranscriptional regulatory mechanism suggests that this keratin may play an important role in designing a cytoskeletal architecture that is compatible with the hyperproliferative state.
角蛋白K6是一种在人类皮肤处于过度增殖状态（如伤口愈合、银屑病和细胞培养）以及恶性转化（如鳞状细胞癌）时表达的蛋白质。当受到诱导时，K6的出现速度很快：如果将皮肤组织置于放射性标记的培养基中，一小时内就能检测到这种蛋白质。K6的调控似乎部分受转录后机制控制：至少有两个K6基因在体内（此时不产生该蛋白质）和体外（此时该蛋白质大量表达）都能被积极转录。通过Northern印迹分析可在皮肤中检测到大量的K6a和K6b RNA，并且这些RNA在体外即使不能完全翻译，也能大量翻译。原位杂交显示这些RNA分布在表皮的整个活性层。通过转录后调控机制快速诱导K6表达表明，这种角蛋白可能在设计与过度增殖状态相适应的细胞骨架结构中发挥重要作用。
