Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, Brazil.
Mycopathologia. 2014 Feb;177(1-2):111-4. doi: 10.1007/s11046-013-9718-0. Epub 2013 Dec 6.
Invasive aspergillosis is a leading cause of morbidity and mortality in immunocompromised patients, particularly in individuals with haematological malignancy and in haematopoietic stem cell transplant recipients. Nowadays, the galactomannan (GM) assay has been widely used as an indication of invasive aspergillosis, even though the test is known to generate false-positive results. The aim of this study was to compare the performance of GM and real-time PCR (qPCR) to detected Aspergillus in blood samples obtained from high-risk haematological patients. Haematological patients were screened twice weekly with GM testing, which was performed by the Platelia ELISA kit. An additional sample of whole blood (4 ml) was obtained for the purpose of qPCR testing. Sixty-four samples from 12 patients with haematopoietic stem cell transplant or haematological malignancy were studied. The overall accordance between GM and qPCR tests was 96.9 % (62 samples). Only two samples showed contradictory results, with positive GM test and negative real-time PCR results. Based on the high concordance between GM and qPCR in terms of negative results, the main utility of qPCR could be in the confirmation of positive results seen with GM testing.
侵袭性曲霉病是免疫功能低下患者发病率和死亡率的主要原因，特别是在血液恶性肿瘤和造血干细胞移植受者中。如今，半乳甘露聚糖（GM）检测已广泛用于侵袭性曲霉病的诊断，尽管该检测已知会产生假阳性结果。本研究旨在比较 GM 和实时 PCR（qPCR）检测血液样本中曲霉的性能，这些血液样本来自高危血液系统疾病患者。通过 Platelia ELISA 试剂盒进行 GM 检测，每周对血液系统疾病患者进行两次筛查。为了进行 qPCR 检测，还采集了 4 毫升全血的额外样本。研究了 12 例造血干细胞移植或血液恶性肿瘤患者的 64 个样本。GM 和 qPCR 检测的总体符合率为 96.9%（62 个样本）。只有两个样本的结果存在差异，GM 检测呈阳性，而实时 PCR 结果为阴性。基于 GM 和 qPCR 在阴性结果方面的高度一致性，qPCR 的主要用途可能是确认 GM 检测呈阳性的结果。
