Department of Agronomy, University of Illinois, 61801, Urbana, Illinois.
J Chem Ecol. 1985 Mar;11(3):383-95. doi: 10.1007/BF01411424.
Relative retention times and wavelength area ratios for over 50 standard compounds were calculated using reverse-phase HPLC. The standard compounds analyzed included benzoic acids, cinnamic acids, benzene carboxylic acids, acetic acids, coumarins, benzaldehydes and a variety of flavonoid compounds including flavanones, flavones, isoflavones, and their glycosides. Each standard compound was chromatographed by three different gradient elutions. Compounds were detected by UV absorption at 254 nm and 280 nm. Relative retention times with respect to two different internal references and the 254nm: 280nm wavelength area ratio was determined for each standard. Soybean root and seed extracts were analyzed for the presence of the standard compounds using the chromatographic conditions described.
使用反相高效液相色谱法计算了 50 多种标准化合物的相对保留时间和波长面积比。分析的标准化合物包括苯甲酸、肉桂酸、苯羧酸、乙酸、香豆素、苯甲醛和多种类黄酮化合物，包括黄烷酮、黄酮、异黄酮及其糖苷。每个标准化合物都通过三种不同的梯度洗脱进行色谱分离。化合物通过在 254nm 和 280nm 处的紫外吸收进行检测。针对每个标准化合物，确定了相对于两个不同内标物的相对保留时间和 254nm：280nm 波长面积比。使用描述的色谱条件分析大豆根和种子提取物中标准化合物的存在情况。
