Xiang Jianfeng, Xiang Yanjie, Lin Shengming, Xin Dongwei, Liu Xiaoyu, Weng Lingling, Chen Tao, Zhang Minguang
Department of Radiology, Shanghai Sixth People's Hospital, Shanghai JiaoTong University, 200233, Shanghai, People's Republic of China.
Tumour Biol. 2014 Apr;35(4):3517-24. doi: 10.1007/s13277-013-1464-x. Epub 2013 Dec 6.
Hepatocellular carcinoma (HCC) is one of the most aggressive malignancies in the world whose chemoprevention became increasingly important in HCC treatment. Although the anticancer effects of asparagus constituents have been investigated in several cancers, its effects on hepatocellular carcinoma have not been fully studied. In this study, we investigated the anticancer effects of the deproteinized asparagus polysaccharide on the hepatocellular carcinoma cells using the in vitro and in vivo experimental model. Our data showed that deproteinized asparagus polysaccharide might act as an effective inhibitor on cell growth in vitro and in vivo and exert potent selective cytotoxicity against human hepatocellular carcinoma Hep3B and HepG2 cells. Further study showed that it could potently induce cell apoptosis and G2/M cell cycle arrest in the more sensitive Hep3B and HepG2 cell lines. Moreover, deproteinized asparagus polysaccharide potentiated the effects of mitomycin both in vitro and in vivo. Mechanistic studies revealed that deproteinized asparagus polysaccharide might exert its activity through an apoptosis-associated pathway by modulating the expression of Bax, Bcl-2, and caspase-3. In conclusion, deproteinized asparagus polysaccharide exhibited significant anticancer activity against hepatocellular carcinoma cells and could sensitize the tumoricidal effects of mitomycin, indicating that it is a potential therapeutic agent (or chemosensitizer) for liver cancer therapy.
肝细胞癌（HCC）是全球最具侵袭性的恶性肿瘤之一，其化学预防在HCC治疗中变得越来越重要。尽管芦笋成分的抗癌作用已在多种癌症中进行了研究，但其对肝细胞癌的影响尚未得到充分研究。在本研究中，我们使用体外和体内实验模型研究了脱蛋白芦笋多糖对肝癌细胞的抗癌作用。我们的数据表明，脱蛋白芦笋多糖可能在体外和体内作为细胞生长的有效抑制剂，并对人肝癌Hep3B和HepG2细胞发挥强大的选择性细胞毒性。进一步研究表明，它可以在更敏感的Hep3B和HepG2细胞系中有效诱导细胞凋亡和G2/M期细胞周期阻滞。此外，脱蛋白芦笋多糖在体外和体内均增强了丝裂霉素的作用。机制研究表明，脱蛋白芦笋多糖可能通过调节Bax、Bcl-2和caspase-3的表达，通过凋亡相关途径发挥其活性。总之，脱蛋白芦笋多糖对肝癌细胞表现出显著的抗癌活性，并能增强丝裂霉素的杀瘤作用，表明它是肝癌治疗的潜在治疗剂（或化学增敏剂）。
