Rodríguez Rocío, Jaramillo Sara, Rodríguez Guillermo, Espejo Juan Antonio, Guillén Rafael, Fernández-Bolaños Juan, Heredia Antonia, Jiménez Ana
Instituto de la Grasa, CSIC, Sevilla, Spain.
J Agric Food Chem. 2005 Jun 29;53(13):5212-7. doi: 10.1021/jf050338i.
Three different methods (antiradical activity, inhibition of primary oxidation, and ferric reducing power) have been used to evaluate the antioxidant activity of eight different asparagus cultivars and byproducts: white and green asparagus from Alcalá del Río (Guadalquivir Valley, Seville) and American hybrids, native spears, and their byproducts from Huétor-Tájar (Vega de Granada). The correlation between antioxidant activity and total phenol content was studied. Six standards were also tested to validate the modified methods for antioxidant activity determination. Results obtained for antiradical capacity and reducing power were very similar, and a high correlation with phenols was found (R > or = 0.9 for both tests). Sample origin was an important factor, spears from Huétor-Tájar having higher values (ARC between 7 and 10 and P(R) of 0.25-0.33) than those from Alcalá del Río (ARC 0.6-2 and P(R) of 0.05-0.07). Significant differences were found between spears with the same origin, suggesting that genetics are another factor to take into account. Asparagus inhibits lipid primary oxidation, but no correlation between the inhibition percentage and phenols was observed. Asparagus origin was the only factor that led to significant differences: samples from Huétor-Tájar had higher values (POIC between 18 and 32) than those from Alcalá del Río (POIC of 5-9). Byproducts from the canning industry at Huétor-Tájar were also assayed for antioxidant activity; the results obtained suggested that byproducts could be considered as an excellent source of natural antioxidants.
采用三种不同方法(抗自由基活性、初级氧化抑制和铁还原能力)评估了八个不同芦笋品种及其副产物的抗氧化活性:来自阿尔卡拉德尔里奥(塞维利亚瓜达尔基维尔河谷)的白芦笋和绿芦笋、美国杂交种、本地芦笋嫩茎及其来自韦托 - 塔哈尔(格拉纳达韦加)的副产物。研究了抗氧化活性与总酚含量之间的相关性。还测试了六种标准物质以验证测定抗氧化活性的改良方法。抗自由基能力和还原能力的结果非常相似,并且发现与酚类物质具有高度相关性(两种测试的R≥0.9)。样品来源是一个重要因素,韦托 - 塔哈尔的芦笋嫩茎具有更高的值(抗自由基能力在7至10之间,还原能力为0.25 - 0.33),高于阿尔卡拉德尔里奥的芦笋嫩茎(抗自由基能力为0.6 - 2,还原能力为0.05 - 0.07)。在相同来源的芦笋嫩茎之间发现了显著差异,这表明遗传因素是另一个需要考虑的因素。芦笋能抑制脂质的初级氧化,但未观察到抑制百分比与酚类物质之间的相关性。芦笋来源是导致显著差异的唯一因素:韦托 - 塔哈尔的样品具有更高的值(初级氧化抑制能力在18至32之间),高于阿尔卡拉德尔里奥的样品(初级氧化抑制能力为5 - 9)。还对韦托 - 塔哈尔罐头工业的副产物进行了抗氧化活性测定;所得结果表明这些副产物可被视为天然抗氧化剂的优质来源。