Subramanian N, Bray M A
J Immunol. 1987 Jan 1;138(1):271-5.
Interleukin (IL 1) preparations from five different sources (human monocyte, LPS-stimulated, purified IL 1 from two different laboratories) and human recombinant IL 1 (HrIL 1) were shown to be capable of directly inducing histamine (HA) release from human basophils (10 to 50% of total cellular HA, depending on the source of IL 1). The release was not due to the medium, pyrogens, or other contaminants. Il 1-induced HA release was dose dependent between 1 to 100 U/ml of IL 1 and 1 to 15 ng/ml of HrIL 1 and was rapid, with a peak release at 15 min. HA release induced by IL 1 was blocked completely by preexposure of cells to IL 1 but was not affected by prechallenge with anti-IgE. Also, preincubation of IL 1 with anti-IL 1 antibody abolished the HA-releasing activity. IL 1-induced HA release was also observed in preparations of human adenoidal mast cells. Our data indicate that it is unlikely that the HA release induced by IL 1 is due to a contamination with HA-releasing factor. This effect of IL 1 provides a mechanism for non-IgE-related local HA release and raises the possibility of a link between cellular immunity and immediate hypersensitivity of potential importance for the pathology of immuno/inflammatory diseases.
来自五种不同来源的白细胞介素（IL-1）制剂（人单核细胞、脂多糖刺激物、来自两个不同实验室的纯化IL-1）和人重组IL-1（HrIL-1）被证明能够直接诱导人嗜碱性粒细胞释放组胺（HA）（释放的HA占细胞总HA的10%至50%，具体取决于IL-1的来源）。这种释放不是由培养基、热原或其他污染物引起的。IL-1诱导的HA释放在IL-1浓度为1至100 U/ml和HrIL-1浓度为1至15 ng/ml之间呈剂量依赖性，且释放迅速，在15分钟时达到释放峰值。IL-1诱导的HA释放可通过细胞预先暴露于IL-1而被完全阻断，但不受抗IgE预激发的影响。此外，IL-1与抗IL-1抗体预孵育可消除HA释放活性。在人腺样体肥大细胞制剂中也观察到了IL-1诱导的HA释放。我们的数据表明，IL-1诱导的HA释放不太可能是由于HA释放因子的污染所致。IL-1的这种作用为非IgE相关的局部HA释放提供了一种机制，并增加了细胞免疫与速发型超敏反应之间存在联系的可能性，这对免疫/炎症性疾病的病理学可能具有重要意义。
