Gibbs B F, Haas H, Falcone F H, Albrecht C, Vollrath I B, Noll T, Wolff H H, Amon U
Department of Dermatology, Medical University of Lübeck, Germany.
Eur J Immunol. 1996 Oct;26(10):2493-8. doi: 10.1002/eji.1830261033.
Recent studies have shown that human basophils, like mast cells, generate interleukin (IL)-4 following immunological activation and may thus participate in late-phase allergic and inflammatory processes. Here, we report the capacity of human basophils to release IL-13 within 24 h following stimulation with anti-IgE. Additionally, in 14 out of 31 experiments, we observed that basophils rapidly release performed IL-4 within 5-10 min, as well as newly generated IL-4, which was released 4 h following stimulation of the cells with anti-IgE. In contrast to the biphasic release of IL-4 from the cells, no preformed IL-13 was detected at earlier times (5-30 min). Preformed IL-4 and IL-4 and IL-13 generated de novo were also released after stimulation of the cells with IL-3; an enhanced production of these cytokines was observed using a combination of IL-3 and anti-IgE. We conclude from these data that, by releasing performed IL-4 and IL-4 and IL-13 generated de novo, human basophils may be centrally involved in the orchestration of allergic inflammation by providing a trigger to IL-4-mediated T helper 2 lymphocyte activation, B cell IgE switching, and increased vascular adhesion molecule expression.
最近的研究表明，人类嗜碱性粒细胞与肥大细胞一样，在免疫激活后会产生白细胞介素（IL）-4，因此可能参与迟发性过敏和炎症过程。在此，我们报告了人类嗜碱性粒细胞在抗IgE刺激后24小时内释放IL-13的能力。此外，在31项实验中的14项中，我们观察到嗜碱性粒细胞在5-10分钟内迅速释放已生成的IL-4，以及新生成的IL-4，后者在抗IgE刺激细胞4小时后释放。与细胞中IL-4的双相释放不同，在较早时间（5-30分钟）未检测到预先形成的IL-13。用IL-3刺激细胞后，预先形成的IL-4以及新生成的IL-4和IL-13也会释放；使用IL-3和抗IgE的组合观察到这些细胞因子的产生增加。从这些数据中我们得出结论，通过释放预先形成的IL-4以及新生成的IL-4和IL-13，人类嗜碱性粒细胞可能通过触发IL-4介导的辅助性T细胞2淋巴细胞激活、B细胞IgE转换和血管黏附分子表达增加，在过敏性炎症的协调中发挥核心作用。
