Department of Biology, University of Virginia, 22901, Charlottesville, VA, U.S.A..
Plant Mol Biol. 1985 Mar;4(2-3):125-32. doi: 10.1007/BF02418759.
We have characterized two related regions of twoPetunia mitochondrial genomes in order to understand how plant mt genomes from a cytoplasmic male sterile (cms) line and a fertile line diverge from one another. Restriction maps of these regions indicate that a sequence arrangement shared by the two genomes adjoins sequences which are not shared at the corresponding locations in the two genomes. A point where the mt genomes from the cms line and the fertile lines diverge from each other was identified and mapped.Previously we had observed that somatic hybrids constructed from the cms and the fertile line contained mt genomes carrying new combinations of parental mtDNA restriction fragments (3). Using the restriction maps of the two related mtDNA regions, a mtDNA arrangement unique to the cms parent could be shown to be present in all 17 stable sterile somatic hybrids tested and none of the 24 stable fertile somatic hybrids tested. This data does not exclude the possibility that additional, as yet unidentified, mtDNA arrangements unique to the cms parent might also be found exclusively in sterile somatic hybrids. Whether or not the sterile parental mtDNA arrangement reported here is functionally related to cms, it apparently segregates with cms in somatic hybrids.
为了了解来自细胞质雄性不育（cms）系和可育系的植物 mt 基因组彼此之间的差异，我们对两个矮牵牛线粒体基因组的两个相关区域进行了特征描述。这些区域的限制图谱表明，两个基因组共享的序列排列与在两个基因组的相应位置不共享的序列相邻。确定并绘制了 cms 系和可育系的 mt 基因组彼此之间分歧的点。此前，我们曾观察到，来自 cms 和可育系的体细胞杂种包含具有双亲 mtDNA 限制片段新组合的 mt 基因组（3）。使用这两个相关 mtDNA 区域的限制图谱，可以证明在所有 17 个稳定不育的体细胞杂种中都存在 cms 亲本特有的 mtDNA 排列，而在 24 个稳定可育的体细胞杂种中均未发现。这些数据并不排除 cms 亲本特有的其他尚未鉴定的 mtDNA 排列也可能仅存在于不育体细胞杂种中的可能性。无论这里报道的不育亲本 mtDNA 排列是否与 cms 具有功能相关性，它显然与体细胞杂种中的 cms 一起分离。
