Temple M, Makaroff C A, Mutschler M A, Earle E D
Department of Plant Breeding and Biometry, Cornell University, Ithaca, NY 14853-1902.
Curr Genet. 1992 Sep;22(3):243-9. doi: 10.1007/BF00351732.
The mitochondrial genomes of nine male-fertile and two Ogura cytoplasmic male-sterile (cms) Brassica napus somatic hybrids were probed with 46 mitochondrial DNA fragments. The distribution of information obtained from each fusion partner was not random. Several regions, including the coxI gene and a major recombination repeat sequence, were always derived from the Brassica campestris fusion partner, and some regions were always derived from the Ogura mitochondrial genome. Novel fragments occurred in seven distinct regions. Some of the rearrangement breakpoints were located near the evolutionary breakpoints relating the mitochondrial genomes of the Brassica species. The sizes of the mitochondrial genomes in the somatic hybrids ranged from 224.8 to 285.3 kb. A direct correlation between a specific gene and the cms phenotype was not observed; however, a possible cms-associated region was identified. It corresponds to a region that was identified through analysis of fertile revertants from a cms B. napus cybrid.
用46个线粒体DNA片段对9个雄性可育和2个奥古拉细胞质雄性不育（cms）甘蓝型油菜体细胞杂种的线粒体基因组进行了探测。从每个融合亲本获得的信息分布并非随机。包括coxI基因和一个主要重组重复序列在内的几个区域总是来自于白菜型油菜融合亲本，而一些区域总是来自于奥古拉线粒体基因组。新片段出现在7个不同区域。一些重排断点位于与芸苔属物种线粒体基因组相关的进化断点附近。体细胞杂种中线粒体基因组的大小在224.8至285.3 kb之间。未观察到特定基因与cms表型之间的直接相关性；然而，确定了一个可能与cms相关的区域。它对应于通过对cms甘蓝型油菜胞质杂种的可育回复体进行分析而确定的一个区域。
