Laboratoire de Virologie, Institut de Biologie Moléculaire et Cellulaire, 15, rue Descartes, 67084, Strasbourg, France.
Plant Mol Biol. 1985 Mar;4(2-3):161-8. doi: 10.1007/BF02418763.
Chromosomal proteins have been isolated from barley (Hordeum vulgare) and corn (Zea mays) nuclei by extraction with 5% perchloric acid. In each plant, one protein was shown to belong to the HMG proteins. Their molecular weights are very close to that of HMG 14 from chicken erythrocytes, as shown by electrophoretic mobility in SDS polyacrylamide gels. In acetic acid-urea-Triton polyacrylamide gels they migrate between HMG 1,2 and HMG 14, from chicken erythrocytes. Their amino acid compositions are typical of HMG proteins, with equivalent high values of acidic and basic residues.Extraction of HMG's from purified barley chromatin fractions with 0.35 M NaCl considerably reduces histone H2 contamination and increases the yield of HMG up to 0.7% of the total histones. In this technique a second protein was extracted which is soluble in 2% Trichloroacetic acid and shows electrophoretic mobility analogous to those of HMG 14 and 17 from chicken erythrocytes. Whether or not these proteins are counterparts of the animal HMG's 1-2 or HMG's 14-17 is discussed.
用 5%高氯酸从大麦（Hordeum vulgare）和玉米（Zea mays）核中提取染色体蛋白。在每种植物中，有一种蛋白被证明属于 HMG 蛋白。其分子量与鸡红细胞中的 HMG14 非常接近，这一点可以通过 SDS 聚丙烯酰胺凝胶中的电泳迁移率显示出来。在乙酸-尿素-Triton 聚丙烯酰胺凝胶中，它们在鸡红细胞的 HMG1、2 和 HMG14 之间迁移。它们的氨基酸组成是典型的 HMG 蛋白，具有相当高的酸性和碱性残基值。用 0.35 M NaCl 从纯化的大麦染色质部分中提取 HMG，可大大减少组蛋白 H2 的污染，并将 HMG 的产量提高到总组蛋白的 0.7%。在这项技术中，还提取了第二种可溶于 2%三氯乙酸的蛋白质，其电泳迁移率类似于鸡红细胞中的 HMG14 和 17。这些蛋白质是否与动物的 HMG1-2 或 HMG14-17 相对应，还有待讨论。
