Yokota Y, Kawata T, Iida Y, Kato A, Tanifuji S
Department of Botany, Faculty of Science, Hokkaido University, Sapporo, Japan.
J Mol Evol. 1989 Oct;29(4):294-301. doi: 10.1007/BF02103617.
Nucleotide sequences of the first and second internal transcribed spacers (ITS1 and ITS2, respectively) of ribosomal DNA (rDNA) from two dicot plants, carrot and broad bean, were determined. These sequences were compared with those of rice, a monocot plant, and other eukaryotic organisms. Both types of ITS region in some species of Angiospermae were the shortest among all eukaryotes so far examined and showed a wide range of variation in their G+C content, in contrast to a general trend toward very high G+C content in animals. Phylogenetic relationships of plants with animals and lower eukaryotes were considered using the nucleotide sequences of carrot and broad bean 5.8S rDNA that were determined in the present study, together with that of wheat 5.8S rRNA, which has been reported previously.
测定了两种双子叶植物胡萝卜和蚕豆核糖体DNA（rDNA）的第一和第二内部转录间隔区（分别为ITS1和ITS2）的核苷酸序列。将这些序列与单子叶植物水稻以及其他真核生物的序列进行了比较。被子植物某些物种的两种ITS区域在目前所检测的所有真核生物中是最短的，并且其G+C含量呈现出广泛的变化，这与动物中G+C含量普遍非常高的趋势形成对比。利用本研究中测定的胡萝卜和蚕豆5.8S rDNA的核苷酸序列，以及先前报道的小麦5.8S rRNA的核苷酸序列，探讨了植物与动物以及低等真核生物之间的系统发育关系。
