Schuster A M, Waddle J A, Korth K, Meints R H
Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331-2906.
Plant Mol Biol. 1990 May;14(5):859-62. doi: 10.1007/BF00016519.
Chloroplast DNA was isolated and cloned from Chlorella, strain N1a, exsymbiotic with Paramecium bursaria. BamHI, SalI, KpnI and Xho I restriction fragments of the DNA were assembled into a circular map. The genome consists of approximately 120 kbp of DNA, has a G/C content of 38%, and contains only a single copy of the rRNA cistron. The rRNA cistron is small, 5000-8000 bp, and the 16S and 23S gene are separated by less than 2000 bp.
从小球藻N1a菌株(与草履虫共生)中分离并克隆了叶绿体DNA。该DNA的BamHI、SalI、KpnI和Xho I限制性片段被组装成一个环状图谱。基因组由约120kbp的DNA组成,G/C含量为38%,并且仅包含rRNA顺反子的单拷贝。rRNA顺反子较小,为5000 - 8000bp,16S和23S基因之间的间隔小于2000bp。