迟发型超敏反应小鼠单核细胞抗原介导的白细胞介素产生
Antigen-mediated interleukin production by mononuclear cells from delayed hypersensitive mice.
作者信息
Kobayashi K, Cohen S, Yoshida T
出版信息
J Exp Pathol. 1985 Fall;2(3):191-209.
Antigen-induced production of interleukins was examined using lymph node cells from BALB/c mice immunized with soluble protein antigens emulsified in complete Freund's adjuvant. We found both Interleukin 1 and Interleukin 2 activity in the antigen-stimulated culture supernatants of lymph node cells obtained from mice immunized with the corresponding antigen. The induction of interleukins correlated with in vivo (footpad response) and in vitro (lymphocyte proliferation) manifestations of delayed hypersensitivity. The kinetics of appearance of IL 1 and IL 2 in culture was similar. Stimulation with unrelated antigens could induce neither interleukins nor both types of cell-mediated immune responses. These results demonstrate that interleukins are inducible by stimulation with soluble proteins under conditions of immunization that lead to cell-mediated immunity.
使用用完全弗氏佐剂乳化的可溶性蛋白抗原免疫的BALB/c小鼠的淋巴结细胞，检测抗原诱导的白细胞介素产生情况。我们在从用相应抗原免疫的小鼠获得的淋巴结细胞的抗原刺激培养上清液中发现了白细胞介素1和白细胞介素2活性。白细胞介素的诱导与体内（足垫反应）和体外（淋巴细胞增殖）迟发型超敏反应表现相关。培养物中IL 1和IL 2出现的动力学相似。用无关抗原刺激既不能诱导白细胞介素，也不能诱导两种类型的细胞介导免疫反应。这些结果表明，在导致细胞介导免疫的免疫条件下，可溶性蛋白刺激可诱导白细胞介素。
Zotero 插件