Peksen Aysun, Kibar Beyhan, Yakupoglu Gokcen
Ondokuz Mayis University, Faculty of Agriculture, Department of Horticulture, 55139 Samsun, Turkey.
Afr J Tradit Complement Altern Med. 2013 Oct 3;10(6):431-4. doi: 10.4314/ajtcam.v10i6.4. eCollection 2013.
In this study, factors such as pH, temperature, carbon and nitrogen sources that affect mycelial growth of Hydnum repandum, a medicinal mushroom, were investigated. Different inoculum media for vegetative inoculum production were also examined. The best suitable pH for mycelial growth was found to be 5.5. Among constant temperatures, the best mycelial growth was obtained at 20 and 25°C. The mycelial growth drastically decreased at 15°C, and no mycelia were obtained at 30°C. Glucose and mannitol were found to be the most suitable carbon sources. Ca(NO3)2 as a nitrogen source gave the best results for mycelial growth. The poorest mycelial growth was noted in sucrose and xylose as carbon sources and in NH4NO3 and (NH4)2HPO4 as nitrogen sources. Peat and peat: vermiculite mixtures (1:4, 1:6, 1:8 and 1:10, v:v) were the best media to use in producing the vegetative inoculum of H. repandum.
在本研究中，对影响药用蘑菇齿菌菌丝体生长的pH值、温度、碳源和氮源等因素进行了研究。还考察了用于生产营养接种体的不同接种培养基。发现菌丝体生长的最适宜pH值为5.5。在恒温条件下，20℃和25℃时菌丝体生长最佳。在15℃时菌丝体生长急剧下降，在30℃时未获得菌丝体。葡萄糖和甘露醇被发现是最适宜的碳源。以Ca(NO3)2作为氮源时，菌丝体生长效果最佳。以蔗糖和木糖作为碳源以及以NH4NO3和(NH4)2HPO4作为氮源时，菌丝体生长最差。泥炭以及泥炭与蛭石的混合物（1:4、1:6、1:8和1:10，体积比）是用于生产齿菌营养接种体的最佳培养基。
