使用萃取纳米电喷雾对超少量未处理样品进行直接质谱分析。
Direct Mass Spectrometry Analysis of Untreated Samples of Ultralow Amounts Using Extraction Nano-Electrospray.
作者信息
Ren Yue, Liu Jiangjiang, Li Linfan, McLuckey Morgan N, Ouyang Zheng
机构信息
Weldon School of Biomedical Engineering, Purdue, West Lafayette, IN 47906.
出版信息
Anal Methods. 2013 Dec 7;5(23). doi: 10.1039/C3AY41149D.
Abstract
Direct mass spectrometry analysis of untreated samples of volumes as low as 0.2 µL were achieved using fast extraction and nanoESI (electrospray ionization) in a combined fashion. The analytes in dried samples on paper substrates were extracted by organic solvent in a nanoESI tube and ionized with a high voltage applied for generating a spray. The ionization source produced stable signals for different atmospheric pressure interfaces of triple quadrupole instruments. Analysis time more than 20 minutes were available with 10 µL solvent consumed for the entire analysis process. The performance in qualitative and quantitative analysis was characterized with a wide variety of samples. Limits of detection as low as 0.1 ng/mL (corresponding to an absolute amount of 0.05 pg) were obtained for analysis of atrazine in river water, thiabendazole in orange homogenate, and methamphetamine in blood.
摘要
采用快速萃取和纳米电喷雾电离（nanoESI）相结合的方式，实现了对低至0.2微升未处理样品的直接质谱分析。纸基质上干燥样品中的分析物通过纳米电喷雾管中的有机溶剂萃取，并用施加的高电压进行电离以产生喷雾。该电离源为三重四极杆仪器的不同大气压接口产生稳定信号。整个分析过程耗时超过20分钟，消耗10微升溶剂。用多种样品对定性和定量分析性能进行了表征。对河水中的阿特拉津、橙汁匀浆中的噻苯达唑和血液中的甲基苯丙胺进行分析时，检测限低至0.1纳克/毫升（对应绝对量为0.05皮克）。
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