黑芥子种子油对链脲佐菌素诱导的糖尿病大鼠降血糖和抗氧化活性的体内评估

In vivo assessment of antihyperglycemic and antioxidant activity from oil of seeds of brassica nigra in streptozotocin induced diabetic rats.

作者信息

Kumar Manoj, Sharma Sunil, Vasudeva Neeru

机构信息

Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Post Box: 38, Hisar-125001, India.

出版信息

Adv Pharm Bull. 2013;3(2):359-65. doi: 10.5681/apb.2013.058. Epub 2013 Aug 20.

DOI:10.5681/apb.2013.058

PMID:24312861

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3848242/

Abstract

PURPOSE

This study was made to investigate the antihyperglycemic and antioxidant potential of oil of seeds of Brassica nigra (BNO) in streptozotocin -nicotinamide (STZ) induced type 2 diabetic rats.

METHODS

BNO was orally administered to diabetic rats to study its effect in both acute and chronic antihyperglycemic study. The body weight, oral glucose tolerance test and biochemical parameters viz. glucose level, insulin level, liver glycogen content, glycosylated hemoglobin and antioxidant parameters were estimated for all treated groups and compared against diabetic control group.

RESULTS

Administration of BNO at a dose 500 mg/kg and 1000 mg/kg body weight p.o. to STZ diabetic rats showed reduction in blood glucose level from 335 mg/dl to 280 mg/dl at 4th h and from 330 mg/dl to 265 mg/dl respectively which was found significant (p<0.01) as compared with diabetic control. BNO (500 mg/kg and 1000 mg/kg) and glibenclamide (0.6 mg/kg) in respective groups of diabetic animals administered for 28 days reduced the blood glucose level in streptozotocin-nicotinamide induced diabetic rats. There was significant increase in body weight, liver glycogen content, plasma insulin level and decrease in glycosylated hemoglobin in test groups as compared to control group. In vivo antioxidant studies on STZ-nicotinamide induced diabetic rat's revealed decreased malondialdehyde (MDA) and increased reduced glutathione (GSH).

CONCLUSION

Thus the results showed that the oil of seeds of Brassica nigra has significant antihyperglycemic and antioxidant activity.

摘要

目的

本研究旨在探讨黑芥子（BNO）种子油对链脲佐菌素-烟酰胺（STZ）诱导的2型糖尿病大鼠的降血糖和抗氧化潜力。

方法

对糖尿病大鼠口服BNO，以研究其在急性和慢性降血糖研究中的作用。对所有治疗组的体重、口服葡萄糖耐量试验和生化参数，即血糖水平、胰岛素水平、肝糖原含量、糖化血红蛋白和抗氧化参数进行评估，并与糖尿病对照组进行比较。

结果

对STZ糖尿病大鼠口服剂量为500 mg/kg和1000 mg/kg体重的BNO后，在第4小时血糖水平分别从335 mg/dl降至280 mg/dl和从330 mg/dl降至265 mg/dl，与糖尿病对照组相比差异有统计学意义（p<0.01）。糖尿病动物各治疗组连续28天分别给予BNO（500 mg/kg和1000 mg/kg）和格列本脲（0.6 mg/kg），可降低链脲佐菌素-烟酰胺诱导的糖尿病大鼠的血糖水平。与对照组相比，试验组的体重、肝糖原含量、血浆胰岛素水平显著增加，糖化血红蛋白降低。对STZ-烟酰胺诱导的糖尿病大鼠进行的体内抗氧化研究显示，丙二醛（MDA）减少，还原型谷胱甘肽（GSH）增加。