三种鼠尾草属植物甲醇提取物的抗伤害感受活性及其对小鼠戒断综合征的影响。

Antinociceptive activity and effect of methanol extracts of three salvia spp. On withdrawal syndrome in mice.

作者信息

Karami Mohammad, Shamerani Mohammad Mehdi, Hossini Ebrahim, Gohari Ahmad Reza, Ebrahimzadeh Mohammad Ali, Nosrati Anahita

机构信息

Department of Toxicopharmacology, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

出版信息

Adv Pharm Bull. 2013;3(2):457-9. doi: 10.5681/apb.2013.075. Epub 2013 Aug 20.

DOI:10.5681/apb.2013.075

PMID:24312878

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3848212/

Abstract

PURPOSE

There are several reports about effects of Salvia spp. on CNS. The present experiment is undertaken to study effect of S. limbata, S. hypoleuca and S. macrosiphon on withdrawal syndrome in mice.

METHODS

Antinociceptive activities of aerial parts of Salvia spp. is investigated using hot plate method. In addition, the effect of its aerial parts on morphine dependence is investigated in mice. After induction of morphine dependency, different concentrations of plant extract are injected. To assess morphine withdrawal, naloxone (5 mg kg-1, i.p.) are injected into mice on the 5th day. Withdrawal syndrome is assessed by placing each mouse in a glass box 30 cm in height and recording the incidence of escape jumps for 60 minutes.

RESULTS

A decrease in incidence of escape jumps is observed in morphine dependence mice. S. limbata and S. hypoleuca extracts produced a statistically significant inhibition of pain induced by hot plate latency at (500, 1000 and 1500 mg kg-1) i.p. A significant increase in pain threshold is observed after 30 and 60 minutes (p < 0.001). The activity was comparable to that of morphine (30 mg kg-1, i.p., p > 0.05). The antinociceptive activity increased up to 60 minutes.

CONCLUSION

S. limbataand S. hypolecuca extracts produced statistically significant inhibition of pain and development of morphine dependence in mice.

摘要

目的

有几篇关于丹参属植物对中枢神经系统作用的报道。本实验旨在研究椭圆叶鼠尾草、地黄鼠尾草和大唇鼠尾草对小鼠戒断综合征的影响。

方法

采用热板法研究丹参属植物地上部分的抗伤害感受活性。此外，还研究了其地上部分对小鼠吗啡依赖性的影响。诱导吗啡依赖后，注射不同浓度的植物提取物。为评估吗啡戒断情况，于第5天给小鼠腹腔注射纳洛酮（5 mg kg-1）。将每只小鼠置于一个30厘米高的玻璃箱中，记录60分钟内逃避跳跃的发生率，以此评估戒断综合征。

结果

在吗啡依赖小鼠中观察到逃避跳跃发生率降低。椭圆叶鼠尾草和地黄鼠尾草提取物在腹腔注射（500、1000和1500 mg kg-1）时，对热板潜伏期诱导的疼痛产生了具有统计学意义的抑制作用。在30分钟和60分钟后观察到疼痛阈值显著升高（p < 0.001）。该活性与吗啡（30 mg kg-1，腹腔注射，p > 0.05）相当。抗伤害感受活性在60分钟内持续增强。