Fujii Tatsuya, Hoshino Tamotsu, Inoue Hiroyuki, Yano Shinichi
Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Higashi-Hiroshima, Hiroshima, Japan.
FEMS Microbiol Lett. 2014 Feb;351(1):32-41. doi: 10.1111/1574-6968.12352. Epub 2013 Dec 18.
The cellulase-producing fungal strain Y-94, isolated in Japan and invalidly described as Acremonium cellulolyticus nom. nud. strain Y-94, seldom forms enteroarthric conidia under nutrient starvation conditions. Phylogenetic analysis using ITS1-5.8S-ITS2 and RNA polymerase II large subunit gene sequences revealed that strain Y-94 is closely related to Talaromyces, given that these Y-94 sequences showed 100% identity with those of Talaromyces pinophilus NBRC 100533 . By contrast, the identity between β-tubulin-encoding genes from strain Y-94 and T. pinophilus NBRC 100533 was 98.1%. Morphological and phenotypic differences between these strains in colony color, conidiophore formation, and cellulase productivity were observed. Together, these data indicated that strain Y-94 belonged to the genus Talaromyces. We propose that strain Y-94 is a new species, Talaromyces cellulolyticus, on the basis of morphology and molecular evidence. The ex-holotype is Y-94 (= FERM BP-5826, CBS 136886 [holotype] TNS-F-48752).
在日本分离得到的产纤维素酶真菌菌株Y-94，曾被错误地描述为无命名的解纤维素顶孢霉（Acremonium cellulolyticus）菌株Y-94，在营养饥饿条件下很少形成关节孢子。利用ITS1-5.8S-ITS2和RNA聚合酶II大亚基基因序列进行的系统发育分析表明，菌株Y-94与嗜松青霉（Talaromyces）密切相关, 因为这些Y-94序列与嗜松青霉NBRC 100533的序列显示出100%的同一性。相比之下，菌株Y-94和解纤维素嗜松青霉NBRC 100533的β-微管蛋白编码基因之间的同一性为98.1%。观察到这些菌株在菌落颜色、分生孢子梗形成和纤维素酶生产力方面的形态和表型差异。综合这些数据表明，菌株Y-94属于嗜松青霉属。基于形态学和分子证据，我们提出菌株Y-94是一个新物种，即解纤维素嗜松青霉（Talaromyces cellulolyticus）。模式菌株为Y-94（=FERM BP-5826，CBS 136886 [模式菌株] TNS-F-48752）。
