一种 dsRNA 真菌病毒，稻瘟病菌 chrysovirus 1-B，抑制了水稻恶苗病菌的营养生长和发育。
A dsRNA mycovirus, Magnaporthe oryzae chrysovirus 1-B, suppresses vegetative growth and development of the rice blast fungus.
机构信息
Laboratories of Molecular and Cellular Biology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo 183-8509, Japan.
出版信息
Virology. 2014 Jan 5;448:265-73. doi: 10.1016/j.virol.2013.10.022. Epub 2013 Nov 5.
A double-stranded RNA (dsRNA) mycovirus was found in isolate S-0412-II 2a of the rice blast fungus Magnaporthe oryzae. Sequence analysis of the five dsRNA segments (dsRNA1 through dsRNA5) revealed that this mycovirus is closely related to Magnaporthe oryzae chrysovirus 1-A (MoCV1-A), tentatively classified as a member of the Chrysoviridae; therefore, it was named Magnaporthe oryzae chrysovirus 1-B (MoCV1-B). Virus particles were spherical and composed of the ORF1, ORF3 and ORF4 proteins. MoCV1-B-infected isolate S-0412-II 2a showed a more severe impaired phenotype than the MoCV1-A-infected isolate. In a virus-cured isolate, normal growth was restored, implied that MoCV1-B could be involved in this observed phenotype. An unanticipated result was the occurrence of a fungal isolate lacking dsRNA5. The nonessential dsRNA5 had higher sequence identity (96%) with dsRNA5 of MoCV1-A than with the other dsRNA segments (71-79%), indicating that dsRNA5 could be a portable genomic element between MoCV1-A and MoCV1-B.
在水稻恶苗病菌 Magnaporthe oryzae 的分离物 S-0412-II 2a 中发现了一种双链 RNA (dsRNA) 真菌病毒。对这五个 dsRNA 片段（dsRNA1 到 dsRNA5）的序列分析表明，这种真菌病毒与 Magnaporthe oryzae chrysovirus 1-A（MoCV1-A）密切相关，暂定为 Chrysoviridae 的成员；因此，它被命名为 Magnaporthe oryzae chrysovirus 1-B（MoCV1-B）。病毒粒子呈球形，由 ORF1、ORF3 和 ORF4 蛋白组成。MoCV1-B 感染的分离物 S-0412-II 2a 表现出比 MoCV1-A 感染的分离物更严重的受损表型。在病毒清除的分离物中，恢复了正常生长，这表明 MoCV1-B 可能参与了这种观察到的表型。一个意外的结果是出现了一种缺乏 dsRNA5 的真菌分离物。非必需的 dsRNA5 与 MoCV1-A 的 dsRNA5 具有更高的序列同一性（96%），而与其他 dsRNA 片段（71-79%）具有更高的序列同一性，这表明 dsRNA5 可能是 MoCV1-A 和 MoCV1-B 之间可移动的基因组元件。
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