Laboratory of Molecular and Cellular Biology, Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, 3-5-8, Saiwaicho, Fuchu, Tokyo, Japan.
The Institute of Environmental Toxicology, 4321, Uchimoriya-machi, Joso, Ibaraki, Japan.
Virology. 2019 Sep;535:241-254. doi: 10.1016/j.virol.2019.07.014. Epub 2019 Jul 15.
A Japanese isolate of Magnaporthe oryzae is infected by Magnaporthe oryzae chrysovirus 1-D (MoCV1-D), which is classified in cluster II of the family Chrysoviridae. The genome of MoCV1-D consists of five dsRNAs. dsRNAs 1-4 show high identity with those of related MoCV1 viruses, whereas dsRNA5 shows relatively low identity and is sometimes deleted during virus propagation. MoCV1-D causes growth inhibition of its host fungus, and the protein encoded by its dsRNA4 impairs cell growth when expressed in yeast cells. It also causes abnormal pigmentation and colony albinization, and we showed that these phenotypes are associated with reduced accumulation of the melanin biosynthesis intermediate scylatone. MoCV1-D exhibits multiform viral structural proteins during prolonged culture. The original host isolate is co-infected with MoCV1-D, a victorivirus, and a partitivirus, and these mycoviruses are detected in cell-free supernatant fractions after prolonged liquid culturing. Hyphal fusion experiments demonstrated that MoCV1-D is transmissible via anastomosis.
一个日本的稻瘟病菌分离株被稻瘟病菌 chrysovirus 1-D（MoCV1-D）感染，该病毒被归类为 chrysoviridae 科的 II 群。MoCV1-D 的基因组由五条 dsRNA 组成。dsRNA1-4 与相关 MoCV1 病毒具有高度同源性，而 dsRNA5 则显示相对较低的同源性，并且在病毒传播过程中有时会缺失。MoCV1-D 会导致其宿主真菌生长受到抑制，其 dsRNA4 编码的蛋白在酵母细胞中表达时会损害细胞生长。它还会导致异常的色素沉着和菌落白化，我们表明这些表型与黑色素生物合成中间产物 scylatone 的积累减少有关。MoCV1-D 在长时间培养过程中表现出多样的病毒结构蛋白。原始宿主分离株与 MoCV1-D、 victorivirus 和 partitivirus 共感染，并且这些真菌病毒在长时间液体培养后可在无细胞上清液中检测到。菌丝融合实验表明 MoCV1-D 可通过吻合进行传播。
